Description

Rh110-2(Asp) [Rhodamine 110, Bis-(L-Aspartic Acid Amide), Trifluoroacetic Acid Salt] is a chemically modified derivative of the classic Rhodamine 110 fluorophore, designed for enhanced water solubility and conjugation capabilities. This compound matters for researchers and manufacturers requiring a bright, green-emitting fluorescent label with a reactive carboxylic acid group for stable covalent attachment to biomolecules. It is primarily needed by the life sciences, diagnostics, and biotechnology industries for developing sensitive assays, probes, and imaging agents.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to peptides, proteins, antibodies, and oligonucleotides via its carboxylic acid group for use in fluorescence-based assays.
• Protease Activity Assays: Serving as a fluorogenic substrate where enzymatic cleavage releases the highly fluorescent Rhodamine 110 core, enabling real-time monitoring of protease activity (e.g., caspases, cathepsins).
• Cell Biology & Live-Cell Imaging: Development of cell-permeable fluorescent probes for tracking intracellular processes, organelle labeling, and monitoring enzymatic activity within living cells.
• Diagnostic Kit Development: Integration into lateral flow assays (LFAs), ELISA, and other diagnostic platforms as a stable, bright signal reporter.
• Flow Cytometry & Fluorescence-Activated Cell Sorting (FACS): As a fluorescent tag for antibody conjugates used in immunophenotyping and cell sorting applications.
• Fluorescence Microscopy: Providing a photostable, green fluorescent signal for confocal, super-resolution, and wide-field microscopy studies.
• Chemical Biology Research: As a tool compound for designing activity-based probes (ABPs) to profile enzyme function in complex biological samples.
• Material Science: Potential use in developing fluorescent sensors, smart polymers, and functionalized nanomaterials.

Basic Information

Product Name	Rh110-2(Asp) [Rhodamine 110, Bis-(L-Aspartic Acid Amide), Trifluoroacetic Acid Salt]
CAS No.	220846-63-9
Molecular Formula	C34H34F3N5O11
Molecular Weight	745.66 g/mol
Synonyms	Rhodamine 110 bis-(L-aspartic acid amide), TFA salt; Bis(L-aspartic acid amide) of Rhodamine 110; Rh110-(Asp)2; Rhodamine 110, N,N'-bis(L-aspartic acid amide); N,N'-Bis(2-amino-1-carboxyethyl)rhodamine 110 trifluoroacetate; Protease Fluorogenic Substrate Precursor; Caspase-3/7 Substrate Derivative; Green Fluorescent Dye-Aspartic Acid Conjugate
EINECS	Contact for details

Quality Control

Our Rh110-2(Asp) is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥ 95.0% (Area % at 254 nm)
Water Content (KF)	≤ 5.0 %
Residual Solvents (GC)	Meets ICH Q3C guidelines
Fluorescence Properties	Ex/Em maxima consistent with Rhodamine 110 derivative

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.