Description

Phalloidin, Rhodamine X Conjugated is a high-purity, fluorescently labeled derivative of the potent bicyclic heptapeptide toxin, phalloidin. This conjugate is specifically engineered for selective and high-affinity staining of filamentous actin (F-actin) in fixed and permeabilized cells, enabling precise visualization of the cytoskeleton. It is an essential research tool for cell biologists, neuroscientists, and toxicologists studying cellular structure, motility, and the mechanisms of cytoskeletal dynamics. The product is supplied as a lyophilized powder, ensuring stability and ease of reconstitution for critical life science applications.

Application

• Fluorescence Microscopy: Primary application for visualizing and quantifying F-actin distribution and organization in fixed cell samples using fluorescence microscopes.
• Cytoskeletal Research: Critical for studies on cell morphology, adhesion, migration, and the structural changes during processes like mitosis and apoptosis.
• Neuroscience Studies: Used to label actin filaments in neuronal growth cones, synapses, and dendritic spines to investigate neural development and plasticity.
• Toxicology & Pharmacology: Serves as a precise probe in studies investigating the effects of toxins, drugs, or genetic modifications on the actin cytoskeleton.
• High-Content Screening (HCS): Employed in automated imaging platforms for phenotypic screening assays that require robust actin staining.
• Multiplex Labeling: Compatible with multi-color immunofluorescence experiments when paired with other probes (e.g., DAPI for nuclei, antibodies for other targets).

Basic Information

Product Name	Phalloidin, Rhodamine X Conjugated
CAS No.	219920-04-4
Molecular Formula	C₆₈H₈₀N₈O₁₆S₂ * (Counterion)ₓ
Molecular Weight	~1400-1500 g/mol (as free acid)
Synonyms	Rhodamine X Phalloidin; TRITC-Phalloidin (related analog); Tetramethylrhodamine B isothiocyanate-Phalloidin Conjugate; Phalloidin-TRITC; F-Actin Probe, Rhodamine conjugate; Actin-staining fluorescent toxin; (3S,6S,9S,12R,15S,18S,21S,24S,27S,33S,36S,39S,42S)-15-[(1S)-1-Hydroxyethyl]-33-[(1R)-1-hydroxy-2-(3-hydroxy-6-oxo-6H-xanthen-9-yl)ethyl]-3,6,9,12,18,21,24,27,30,36,39,42-dodecaazatricyclo[42.3.0.0¹⁵,²⁷]heptatetracontane-1,4,7,10,13,16,19,22,25,28,31,34,37,40-tetradecaone
EINECS	Contact for details

Quality Control

Every batch of Phalloidin, Rhodamine X Conjugated is manufactured and tested under stringent quality management systems. The product undergoes comprehensive analytical characterization, including HPLC for purity assessment and spectroscopic methods for conjugate verification, to ensure it meets the exacting standards required for reproducible research. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, fluorescence performance, and functional activity in a standardized F-actin staining assay.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep the lyophilized powder at -20°C in a desiccated environment. After reconstitution, prepare aliquots and store the working solution at -20°C, protected from light, to minimize freeze-thaw cycles and maintain optimal fluorescence intensity. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Red to reddish-brown lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~92,000 cm⁻¹M⁻¹ (at λmax ~580 nm)
Fluorescence Emission	Maximum ~602 nm (in methanol)
Functional Activity (Staining)	Produces intense, specific F-actin labeling in standardized cell assays
Solubility	Soluble in methanol, DMSO, and aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.