Description

Dhr 6G (Dihydrorhodamine 6G) is a reduced, non-fluorescent derivative of the classic fluorescent dye Rhodamine 6G, widely used as a critical reagent in oxidative stress research. Its core value lies in its specific reaction with reactive oxygen species (ROS), such as hydrogen peroxide, to regenerate the highly fluorescent Rhodamine 6G, enabling sensitive detection and quantification. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and chemical manufacturing sectors, where monitoring oxidative processes is vital for product stability and biological studies.

Application

• Biomarker for Oxidative Stress: A key substrate in assays to detect and quantify intracellular reactive oxygen species (ROS) like hydrogen peroxide and peroxynitrite.
• Pharmaceutical R&D: Used in high-throughput screening to evaluate the antioxidant properties of new drug candidates and study drug-induced oxidative stress.
• Cell Biology & Toxicology: Employed in fluorescence microscopy and flow cytometry to assess oxidative burst in immune cells and cellular health under toxicological stress.
• Material Science: Acts as a probe in studying oxidative degradation and stability of polymers, coatings, and other advanced materials.
• Environmental Monitoring: Utilized in developing sensitive assays for detecting oxidative pollutants in water and air samples.
• Diagnostic Kit Manufacturing: Serves as a core component in commercial diagnostic kits designed for oxidative stress measurement in clinical and research settings.

Basic Information

Product Name	Dhr 6G (Dihydrorhodamine 6G)
CAS No.	217176-83-5
Molecular Formula	C28H31N2O3
Molecular Weight	443.56 g/mol
Synonyms	Dihydrorhodamine 6G; DHR 6G; DHR6G; Reduced Rhodamine 6G; 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester; N-(9-(2-Carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene)-N-ethylethanaminium chloride (reduced form); DHR; ROS probe DHR 6G
EINECS	Contact for details

Quality Control

Our Dihydrorhodamine 6G is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is light-sensitive and should be handled under appropriate conditions to prevent premature oxidation and degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Fluorescence Response	Validated per internal protocol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.