Description

Dcdhfda, Se [2',7'-Dichlorodihydrofluorescein Diacetate, Succinimidyl Ester] is a cell-permeable, non-fluorescent probe designed for the sensitive detection of intracellular reactive oxygen species (ROS). This compound matters as a critical research tool, enabling the quantitative measurement of oxidative stress in live cells through its conversion to a highly fluorescent product. It is essential for researchers and quality control labs in the pharmaceutical, biotechnology, and life sciences industries who require reliable and specific assays for cellular health and drug mechanism studies.

Application

• Intracellular ROS Detection: Primary application for measuring hydrogen peroxide, peroxynitrite, and other reactive oxygen species in live-cell imaging and flow cytometry.
• Oxidative Stress Assays: Used in high-throughput screening (HTS) platforms to evaluate the pro-oxidant or antioxidant effects of novel drug candidates and natural compounds.
• Neurodegenerative Disease Research: A key reagent in studies investigating oxidative damage mechanisms in models of Alzheimer's, Parkinson's, and other neurological disorders.
• Cancer Biology Studies: Employed to monitor ROS levels associated with chemotherapy, radiotherapy, and altered metabolism in cancer cell lines.
• Immunology and Inflammation Research: Facilitates the study of ROS production in immune cells like macrophages and neutrophils during inflammatory responses.
• Toxicology and Environmental Testing: Applied in cell-based assays to assess the oxidative potential and cytotoxicity of industrial chemicals and environmental pollutants.

Basic Information

Product Name	Dcdhfda, Se [2',7'-Dichlorodihydrofluorescein Diacetate, Succinimidyl Ester]
CAS No.	216964-01-1
Molecular Formula	C29H19Cl2NO10
Molecular Weight	612.37 g/mol
Synonyms	2',7'-Dichlorodihydrofluorescein diacetate, succinimidyl ester; DCFH-DA, SE; H2DCFDA, SE; Dichlorodihydrofluorescein diacetate, N-succinimidyl ester; 2',7'-Dichloro-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate, 2,5-dioxopyrrolidin-1-yl ester; ROS probe DCFH-DA SE; CellROX precursor reagent; Intracellular ROS indicator, SE-activated.
EINECS	Contact for details

Quality Control

Our Dcdhfda, Se is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot undergoes rigorous analytical testing, including HPLC purity analysis and functional validation in cell-based assays. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Functional Activity (Cell Assay)	Conforms to reference standard
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.