Description

6-Carboxyrhodamine 6G, Succinimidyl Ester is a reactive, amine-reactive fluorescent labeling reagent widely used in bioanalytical chemistry. This compound is critical for creating highly sensitive and stable fluorescent conjugates with proteins, peptides, and other biomolecules. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for applications in fluorescence microscopy, flow cytometry, and immunoassays.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to primary amines (-NH₂) on antibodies, proteins, peptides, and oligonucleotides for detection and imaging.
• Diagnostic Assay Development: Used as a key component in the synthesis of fluorescent probes and tracers for ELISA, lateral flow assays, and other in vitro diagnostic (IVD) kits.
• Cell Biology & Microscopy: Preparation of fluorescently-tagged ligands, substrates, or nanoparticles for live-cell imaging, intracellular tracking, and confocal microscopy studies.
• Flow Cytometry: Creation of bright, photostable antibody-dye conjugates for multicolor panel design in immunophenotyping and cell sorting.
• Proteomics & Biomarker Research: Labeling of proteins for gel-based fluorescence detection (e.g., 2D-DIGE) or for capillary electrophoresis and HPLC analysis.
• Materials Science: Functionalization of surfaces, polymers, or nanomaterials with fluorescent tags for sensor development and material characterization.

Basic Information

Product Name	6-Carboxyrhodamine 6G, Succinimidyl Ester
CAS No.	216699-37-5
Molecular Formula	C34H31N3O7
Molecular Weight	593.63 g/mol
Synonyms	6-Carboxy-X-rhodamine (6-ROX) succinimidyl ester; 6-ROX, SE; 6-Carboxyrhodamine 6G NHS ester; Rhodamine 6G carboxylic acid succinimidyl ester; 5(6)-Carboxyrhodamine 6G, N-hydroxysuccinimide ester; X-Rhodamine NHS ester; C34H31N3O7; N-Succinimidyl 6-carboxyrhodamine 6G ester
EINECS	Contact for details

Quality Control

Our 6-Carboxyrhodamine 6G, Succinimidyl Ester is manufactured under strict quality systems to ensure high purity and consistent performance for sensitive applications. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and functional performance is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active ester.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	575 - 585 nm
Emission Maximum	595 - 605 nm
Extinction Coefficient (ε)	≥ 80,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 1.0%
Functional Performance	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.