Description

5-Carboxy-X-Rhodamine Triethylamine Salt is a high-purity, water-soluble fluorescent dye derivative, specifically designed for advanced fluorescence applications. This compound is critical for researchers and developers requiring a bright, photostable, and reactive label for biomolecular conjugation and detection. It is an essential reagent for professionals in the life sciences, analytical chemistry, and diagnostic industries who demand reliable and consistent fluorescent probes for sensitive assays.

Application

• Fluorescent Labeling of Biomolecules: Primary use for covalently labeling proteins, peptides, antibodies, and nucleic acids (e.g., DNA, RNA) via its carboxylic acid functional group.
• Fluorescence Microscopy & Imaging: Used as a vital stain or tag in cell biology for confocal, super-resolution, and live-cell imaging due to its excellent photophysical properties.
• Flow Cytometry (FACS): Employed in the preparation of fluorescently-labeled antibodies and other probes for multiparameter cell analysis and sorting.
• Fluorescence In Situ Hybridization (FISH): Acts as a bright fluorophore for labeling nucleic acid probes in genetic testing and cytogenetics.
• Diagnostic Assay Development: Integral component in the manufacture of fluorescent lateral flow assays, ELISA kits, and other point-of-care diagnostic devices.
• Sensor & Probe Fabrication: Key material for developing chemical sensors and biosensors that rely on fluorescence signal transduction.
• Material Science Research: Used in the development of fluorescent nanomaterials, polymers, and coatings for tracking and imaging applications.

Basic Information

Product Name	5-Carboxy-X-Rhodamine Triethylamine Salt, For Fluorescence*
CAS No.	216699-35-3
Molecular Formula	C34H35N3O7 • C6H15N
Molecular Weight	~733.86 g/mol (for the salt form)
Synonyms	5-Carboxy-X-rhodamine, triethylammonium salt; 5-ROX, SE (triethylamine salt); X-Rhodamine-5-carboxylic acid, triethylamine salt; 5-Carboxy-X-rhodamine N-succinimidyl ester, TEA salt (common precursor form); 5-Carboxy-X-rhodamine, TEA salt; ROX-5 acid, TEA salt; CXR, TEA salt; [5-(and-6)-Carboxy-X-rhodamine] triethylamine salt
EINECS	Contact for details

Quality Control

Our 5-Carboxy-X-Rhodamine Triethylamine Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive fluorescence applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, spectrophotometric analysis for absorbance/fluorescence properties, and moisture content determination. A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive). For near-term use, the unopened vial may be stored desiccated at 2-8°C. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	575 - 585 nm
Emission Maximum	600 - 610 nm
Moisture Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.