Description

Cy5.5 is a near-infrared (NIR) cyanine dye widely recognized for its exceptional fluorescence properties. This compound is crucial for enabling high-sensitivity detection and imaging in advanced biomedical research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, in vivo imaging, and flow cytometry who require deep tissue penetration and minimal background interference.

Application

• Bioconjugation and Probe Labeling: Conjugation to antibodies, peptides, proteins, and oligonucleotides for the development of fluorescent probes and detection kits.
• In Vivo Imaging: Used as a contrast agent for non-invasive fluorescence imaging in small animal studies, offering deep tissue penetration in the NIR window.
• Flow Cytometry & Cell Analysis: Labeling of cells for multiplexed analysis and detection using NIR laser channels.
• Microscopy: Fluorescence labeling for fixed and live-cell imaging applications in confocal and super-resolution microscopy.
• Diagnostic Assay Development: Key component in the development of lateral flow assays, ELISA, and other immunoassays requiring NIR detection.
• Drug Delivery & Pharmacokinetics: Tagging of nanoparticles, liposomes, and therapeutic molecules to track biodistribution and targeting efficacy.

Basic Information

Product Name	Cy5.5
CAS No.	210892-23-2
Molecular Formula	C44H47N3O9S22+ • 2X- (X = counterion)
Molecular Weight	~853.99 g/mol (Free acid form)
Synonyms	Cyanine5.5; Cy 5.5; Sulfo-Cyanine5.5; Cy5.5 NHS ester; 1H-Benz[e]indolium, 2-[5-[1,1-dimethyl-3-(4-sulfobutyl)-1,3-dihydro-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1-dimethyl-3-(4-sulfobutyl)-, inner salt; IRDye® 800CW related dye; NIR dye 5.5
EINECS	Contact for details

Quality Control

Our Cy5.5 is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and functional testing for labeling efficiency. Certificates of Analysis (COA) detailing batch-specific results are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	675 - 685 nm
Emission Maximum (λem)	695 - 710 nm
Extinction Coefficient (ε)	≥ 190,000 cm-1M-1
Moisture Content (KF)	≤ 5.0 %
Functional Performance (Labeling)	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.