Description

Cy5.5 Acid (Tri So3) is a water-soluble, near-infrared (NIR) fluorescent dye derivative functionalized with three sulfonate groups and a carboxylic acid. This compound is essential for creating stable, bright, and biocompatible conjugates for advanced diagnostic and research applications. It is primarily needed by biotechnology, pharmaceutical, and medical diagnostics companies developing in vivo imaging agents, flow cytometry reagents, and fluorescent probes for high-sensitivity detection.

Application

• Bioconjugation and Probe Synthesis: The carboxylic acid group enables covalent labeling of antibodies, peptides, proteins, and nanoparticles for NIR fluorescence applications.
• In Vivo Imaging: Used to label targeting molecules for non-invasive optical imaging in preclinical research, leveraging its deep tissue penetration and low background autofluorescence.
• Flow Cytometry & Microscopy: Serves as a critical component in developing high-performance fluorescent reagents and dyes for cell analysis and subcellular imaging.
• Diagnostic Assay Development: Integral for creating sensitive fluorescent immunoassays (FLISA) and lateral flow test strips.
• Material Science: Can be incorporated into polymers and coatings to create smart materials with fluorescent properties.

Basic Information

Product Name	Cy5.5 Acid (Tri So3)
CAS No.	210834-26-7
Molecular Formula	C44H47N3O14S33- • 3Na+ (sodium salt form)
Molecular Weight	Approx. 959.0 g/mol (Free acid) / 1024.9 g/mol (Trisodium salt)
Synonyms	Sulfo-Cyanine5.5 carboxylic acid; Cyanine5.5 trisulfonate acid; Cy5.5 Tri-Sulfonated Acid; 1H-Benz[e]indolium, 2-[5-[1-carboxy-5-[(1E,3E)-5-[1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benz[e]indol-2-ylidene]-1,3-pentadienyl]-2,3-dihydro-1H-indol-2-yl]ethenyl]-1,1-dimethyl-3-(4-sulfonatobutyl)-, inner salt, sodium salt; NIR Dye Acid; Sulfo-Cy5.5 COOH
EINECS	Contact for details

Quality Control

Our Cy5.5 Acid (Tri So3) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and mass spectrometry for identity confirmation. Certificates of Analysis (COA) with detailed specifications are provided to ensure consistency and performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	680 - 690 nm
Emission Maximum (λem)	700 - 710 nm
Extinction Coefficient (ε)	≥ 190,000 cm-1M-1
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.