Description

5-Carboxyrhodamine 6G Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent widely used in bioanalytical chemistry. This compound is critical for creating stable, fluorescent conjugates with proteins, antibodies, and other biomolecules, enabling sensitive detection and analysis. It is an essential tool for researchers and manufacturers in the fields of flow cytometry, immunoassays, fluorescence microscopy, and nucleic acid detection.

Application

• Fluorescent Labeling of Proteins and Antibodies: Primary application for creating probes used in immunofluorescence (IF), Western blotting, and ELISA.
• Flow Cytometry Reagent: Used to label cell surface markers and intracellular proteins for multi-color flow cytometric analysis.
• Nucleic Acid Labeling: Conjugation to oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Microscopy and Cell Imaging: Preparation of fluorescent tags for live-cell and fixed-cell imaging studies.
• Diagnostic Kit Manufacturing: A key component in the production of fluorescent lateral flow assays and other clinical diagnostic kits.
• Bioconjugation and Chemical Biology: Used in research for studying protein-protein interactions, receptor labeling, and developing novel therapeutic conjugates.

Basic Information

Product Name	5-Carboxyrhodamine 6G Succinimidyl Ester
CAS No.	209112-21-0
Molecular Formula	C34H31N3O7
Molecular Weight	593.63 g/mol
Synonyms	6-Carboxy-X-rhodamine succinimidyl ester; 6-ROX NHS ester; 5(6)-Carboxyrhodamine 6G N-hydroxysuccinimide ester; X-Rhodamine NHS Ester; 6-Carboxyrhodamine 6G, succinimidyl ester; CXR 6G SE; Rhodamine 6G carboxylic acid succinimidyl ester
EINECS	Contact for details

Quality Control

Our 5-Carboxyrhodamine 6G Succinimidyl Ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is characterized by HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~575 nm
Emission Maximum	~602 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.