Description

Rhodamine 123 Hydrate is a fluorescent cationic dye widely used as a selective mitochondrial stain in live cells. This compound is valued for its high photostability and low cytotoxicity, making it an essential tool for probing mitochondrial membrane potential and cell viability. It is primarily utilized by researchers and quality control laboratories in the biotechnology, pharmaceutical, and life sciences sectors for applications in flow cytometry, fluorescence microscopy, and apoptosis studies.

Application

• Mitochondrial Staining: Selective staining of mitochondria in live cells for fluorescence microscopy and imaging.
• Flow Cytometry: Assessment of mitochondrial membrane potential and cell health in high-throughput analysis.
• Apoptosis Research: Detection of early-stage apoptotic cells by monitoring changes in mitochondrial function.
• Cell Viability Assays: Used as a fluorescent marker to distinguish between viable and non-viable cell populations.
• P-glycoprotein (P-gp) Studies: Employed as a substrate in functional assays to study multidrug resistance in cancer cells.
• Neuroscience Research: Labeling of neuronal mitochondria to study bioenergetics and neurodegenerative processes.

Basic Information

Product Name	Rhodamine 123 Hydrate
CAS No.	207671-45-2
Molecular Formula	C21H17ClN2O3 • xH2O
Molecular Weight	380.83 (Anhydrous basis)
Synonyms	Rhodamine 123; 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester hydrochloride hydrate; Basic Violet 10; R123; Rh 123; Rhodamine 123 chloride; 3,6-Diamino-9-[2-(methoxycarbonyl)phenyl]xanthylium chloride hydrate; Mitochondrial Green
EINECS	Contact for details

Quality Control

Our Rhodamine 123 Hydrate is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopy for identity confirmation, and residual solvent analysis. A Certificate of Analysis (COA) detailing the specific results for purity, appearance, and other critical parameters is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at room temperature (15-25°C) in a cool, dry place. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Fluorescence	Conforms (Ex/Em ~507/529 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.