Description

4,5-Diaminofluorescein is a highly reactive and versatile fluorescent dye derivative, primarily valued for its role as a key intermediate in the synthesis of advanced fluorescent probes and sensors. Its significance lies in its ability to act as a precursor for creating sensitive detection reagents used in biochemical and analytical applications. This compound is essential for manufacturers and researchers in the life sciences, diagnostics, and analytical chemistry sectors who require high-purity building blocks for developing novel detection systems.

Application

• Fluorescent Probe Synthesis: A critical intermediate for manufacturing reactive fluorescent dyes and tags used in bio-conjugation.
• Biochemical Assays: Used in the development of sensitive detection kits for enzymes, metal ions, and reactive oxygen species.
• Cell Biology Research: Serves as a precursor for cell-permeable dyes used in live-cell imaging and intracellular sensing.
• Diagnostic Reagents: Employed in the production of reagents for clinical diagnostics and point-of-care testing devices.
• Material Science: Can be used to create fluorescent polymers or coatings for sensor applications.
• Analytical Chemistry: Acts as a derivatization agent for the HPLC or capillary electrophoresis analysis of specific analytes.

Basic Information

Product Name	4,5-Diaminofluorescein
CAS No.	205391-01-1
Molecular Formula	C20H14N2O5
Molecular Weight	362.34 g/mol
Synonyms	4,5-Diaminofluorescein; 4',5'-Diaminofluorescein; 2-(3,4-Diamino-6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid; 4,5-DAF; DAF; Fluorescein-4,5-diamine; 9-(o-Carboxyphenyl)-4,5-diamino-6-hydroxy-3H-xanthen-3-one
EINECS	Contact for details

Quality Control

Our 4,5-Diaminofluorescein is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and manufacturing applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to minimize exposure to moisture and air.

Specification

Item	Specification
Appearance	Orange to red-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.