Description

n-[12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoyl]-Ceramide is a specialized fluorescently labeled sphingolipid derivative, designed for advanced research applications. Its core value lies in the incorporation of the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) fluorophore, enabling precise tracking and visualization within complex biological systems. This compound is essential for researchers in lipidomics, cell biology, and membrane biophysics who require high-purity, well-characterized probes to study ceramide trafficking, metabolism, and signaling pathways.

Application

• Fluorescent Labeling & Cellular Imaging: As a vital tool for tracing the intracellular localization, transport, and metabolism of ceramides using fluorescence microscopy (e.g., confocal, TIRF).
• Lipid Raft & Membrane Dynamics Studies: Used to investigate the behavior and partitioning of ceramides within lipid bilayers and membrane microdomains.
• Sphingolipid Metabolic Pathway Analysis: Serves as a substrate or tracer in enzymatic assays to study ceramide-related synthesis and catabolism.
• Apoptosis & Cell Signaling Research: Enables visualization of ceramide generation and accumulation during programmed cell death and other signaling events.
• Model Membrane Systems: Incorporation into liposomes or supported lipid bilayers (SLBs) for in vitro biophysical studies.
• High-Content Screening (HCS): Can be utilized in automated imaging platforms for drug discovery targeting sphingolipid pathways.
• Standards for Analytical Chemistry: Acts as a critical reference standard for LC-MS or HPLC-based quantification of ceramide species in biological samples.

Basic Information

Product Name	n-[12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoyl]-Ceramide
CAS No.	202850-01-9
Molecular Formula	C52H90N6O8
Molecular Weight	927.32 g/mol
Synonyms	NBD C12-Ceramide; NBD-Ceramide (dodecanoyl); N-[12-[(7-Nitro-4-benzofurazanyl)amino]dodecanoyl]-D-erythro-sphingosine; N-(12-((7-Nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)dodecanoyl)-D-erythro-sphingosine; Fluorescent Ceramide NBD C12; Ceramide NBD; NBD labeled Ceramide; C12-NBD-Ceramide
EINECS	Contact for details

Quality Control

Our NBD C12-Ceramide is produced under stringent conditions to ensure batch-to-batch consistency and high chemical purity, critical for sensitive biological assays. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and fluorescence spectroscopy to verify performance. Certificates of Analysis (COA) detailing all specifications are provided to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to light, moisture, or elevated temperatures will degrade the fluorescent label and reduce product performance. Aliquot upon receipt to minimize freeze-thaw cycles.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in DMSO, ethanol, DMF
Extinction Coefficient (ε)	~21,000 cm-1M-1 (in methanol, ~467 nm)
Fluorescence Emission	~534 nm (in membrane environments)
Water Content (KF)	< 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.