Description

3',5'-o-[1,1,3,3-Tetrakis(1-Methylethyl)-1,3-Disiloxanediyl]Uridine 2'-(4-Nitrophenyl Carbonate) is a sophisticated, high-purity nucleoside derivative designed for advanced synthetic applications. This compound is a critical building block for the synthesis of modified oligonucleotides, enabling precise control over nucleic acid structure and function. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel therapeutics, diagnostic probes, and advanced materials science.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of RNA and modified DNA strands.
• Antisense & siRNA Therapeutics: Used in the research and production of therapeutic oligonucleotides for gene silencing and targeted therapies.
• Diagnostic Probe Development: Essential for creating labeled probes for fluorescence in situ hybridization (FISH) and other molecular diagnostic assays.
• Aptamer Research: Serves as a modified monomer in the selection and synthesis of nuclease-resistant aptamers.
• Bioconjugation: The reactive 2'-O-(4-nitrophenyl carbonate) group facilitates site-specific conjugation to other molecules, such as fluorophores or targeting ligands.
• Academic & Industrial R&D: A vital reagent for fundamental research in nucleic acid chemistry and biochemistry.

Basic Information

Product Name	3',5'-o-[1,1,3,3-Tetrakis(1-Methylethyl)-1,3-Disiloxanediyl]Uridine 2'-(4-Nitrophenyl Carbonate)
CAS No.	202533-73-1
Molecular Formula	C30H45N3O10Si2
Molecular Weight	663.87 g/mol
Synonyms	2'-O-(4-Nitrophenylcarbonate)-3',5'-O-(1,1,3,3-tetraisopropyldisiloxane-1,3-diyl)uridine; 2'-O-(4-Nitrophenyloxycarbonyl)-3',5'-O-(TIPDS)uridine; TIPDS-Protected Uridine 2'-(4-Nitrophenyl Carbonate); Uridine, 3',5'-O-[1,1,3,3-tetrakis(1-methylethyl)-1,3-disiloxanediyl]-, 2'-(4-nitrophenyl) carbonate; 2'-O-(p-Nitrophenyloxycarbonyl)-3',5'-O-(1,1,3,3-tetraisopropyldisiloxan-1,3-diyl)uridine; 5'-O-TIPDS-2'-O-(4-nitrophenyloxycarbonyl)uridine
EINECS	Contact for details

Quality Control

Our production of this high-value intermediate adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive synthetic workflows. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, NMR spectroscopy for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.