Description

Py-Bodipy-NHS Ester is a highly reactive, amine-reactive fluorescent labeling reagent derived from the versatile BODIPY (boron-dipyrromethene) fluorophore core. This compound is specifically engineered for the efficient and stable conjugation to primary amines, such as those on proteins, peptides, antibodies, and other biomolecules, facilitating the creation of bright, photostable fluorescent probes. It is an essential tool for researchers and developers in bioconjugation, bioimaging, flow cytometry, and diagnostic assay development, where precise and durable labeling is critical.

Application

• Bioconjugation and Probe Synthesis: Covalent labeling of proteins, antibodies, peptides, and oligonucleotides for creating fluorescent detection reagents.
• Bioimaging and Microscopy: Preparation of fluorescent tags for cellular and tissue imaging applications, including confocal and super-resolution microscopy, due to its excellent photostability.
• Flow Cytometry: Conjugation to antibodies or other targeting molecules for high-sensitivity cell surface marker detection and analysis.
• Diagnostic Assay Development: Used in the manufacture of fluorescent lateral flow assays (LFAs), ELISA kits, and other in-vitro diagnostic (IVD) components.
• Chemical Biology and Drug Discovery: Labeling of small molecules or biomolecules to study biological pathways, target engagement, and pharmacokinetics.
• Material Science: Functionalization of nanoparticles, polymers, or surfaces with fluorescent properties for sensing and tracking applications.

Basic Information

Product Name	Py-Bodipy-NHS Ester
CAS No.	201998-61-0
Molecular Formula	C24H22BF2N3O5
Molecular Weight	481.26 g/mol
Synonyms	BODIPY FL NHS Ester; 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionic Acid, Succinimidyl Ester; Py-BODIPY® SE; BDP FL NHS ester; BODIPY® FL, SE; 3-(2,4-Dimethylpyrrol-5-yl)methylidene-2-methyl-1-(N-succinimidyl)-pyrrole boron difluoride
EINECS	Contact for details

Quality Control

Our Py-Bodipy-NHS Ester is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A Certificate of Analysis (COA) detailing the specific results for identity, purity, and performance is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~80,000 cm-1M-1 at approx. 504 nm
Emission Maximum	~511 nm
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.