Description

Baclight(Tm) Green Bacterial Stain is a vital fluorescent nucleic acid stain specifically designed for the selective detection and enumeration of bacteria. This compound offers a critical solution for researchers and quality control professionals requiring rapid, reliable bacterial viability assessment. It is essential for industries and laboratories focused on microbiology, pharmaceutical sterility testing, and environmental monitoring.

Application

• Bacterial Viability and Cell Counting: Primary application for distinguishing between live and dead bacterial cells in suspension using fluorescence microscopy or flow cytometry.
• Pharmaceutical Sterility Testing: Used in rapid microbiological methods (RMM) for monitoring contamination in cleanrooms, water systems, and final product batches.
• Environmental and Water Quality Monitoring: Detection and quantification of bacterial load in water samples, biofilms, and industrial process streams.
• Food and Beverage Safety: Application in hygiene monitoring and microbial contamination screening for production surfaces and ingredients.
• Clinical and Diagnostic Research: Tool for studying bacterial infection mechanisms, antibiotic susceptibility testing, and host-pathogen interactions.
• Biotechnology and Fermentation: Monitoring bacterial cell culture health and density during upstream bioprocessing.

Basic Information

Product Name	Baclight(Tm) Green Bacterial Stain
CAS No.	201860-17-5
Molecular Formula	C37H47N3O8S2+
Molecular Weight	~701.91 g/mol (for cation)
Synonyms	SYTO 9; BacLight Green Stain; Cell-permeant nucleic acid stain; 1H-Benz[e]indolium, 2-[4-(diethylamino)phenyl]ethenyl]-1,3-dimethyl-, salt with 4-methylbenzenesulfonic acid (1:1); Green fluorescent nucleic acid stain; Live/Dead BacLight component; Bacterial viability stain
EINECS	Contact for details

Quality Control

Every batch of Baclight(Tm) Green Bacterial Stain is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure performance consistency for critical research and industrial applications. A Certificate of Analysis (COA) detailing purity, functional performance in standard assays, and absence of interfering impurities is available for each lot.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. For prolonged storage, consider desiccation.

Specification

Item	Specification
Appearance	Dark green to greenish-black solid or solution
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Functional Performance	Yields distinct fluorescence signal for bacterial nucleic acids as per reference method
Solubility	Soluble in DMSO, DMF, ethanol
Moisture Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.