Description

Alexa Fluor 350 is a bright blue-fluorescent, water-soluble, sulfonated dye belonging to the Alexa Fluor series of superior fluorescent labels. This product is specifically engineered for high performance in demanding fluorescence applications, offering exceptional photostability and a high quantum yield. It is an essential tool for researchers and industrial scientists in life sciences, diagnostics, and biotechnology who require reliable and consistent fluorescent labeling for microscopy, flow cytometry, and nucleic acid detection.

Application

• Fluorescence Microscopy & Imaging: Conjugation to antibodies, streptavidin, or other proteins for cellular and tissue imaging.
• Flow Cytometry (FACS): Used as a fluorescent tag for cell surface marker detection and analysis.
• Nucleic Acid Labeling & Detection: Labeling of oligonucleotides, DNA probes, and in situ hybridization (FISH) applications.
• Immunoassays & Diagnostics: Development of fluorescent immunoassays (e.g., ELISA) and diagnostic test kits.
• Fluorescence Resonance Energy Transfer (FRET): Serving as a donor dye in FRET-based molecular sensing and interaction studies.
• Western Blotting: Conjugation to secondary antibodies for highly sensitive protein detection on blots.

Basic Information

Product Name	Alexa Fluor 350
CAS No.	200554-19-4
Molecular Formula	C22H21N3Na2O9S3
Molecular Weight	629.58 g/mol
Synonyms	Alexa Fluor™ 350; Alexa Fluor 350 carboxylic acid, trisodium salt; 6-((((2-((3-Carboxy-4-((2,5-dioxo-1-pyrrolidinyl)oxy)phenyl)amino)-1,3,5-triazin-6-yl)amino)methyl)-2,7-disulfo-1H,5H-benzo[ij]quinolizin-9-ylidene)methyl)-1-ethyl-4-(hydroxymethyl)quinolin-1-ium, inner salt, trisodium salt; Alexa Fluor® 350 SE; Succinimidyl ester of Alexa Fluor 350; AF350; AF 350
EINECS	Contact for details

Quality Control

Our Alexa Fluor 350 is produced under stringent quality management systems to ensure batch-to-batch consistency and performance. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and labeling functionality. A comprehensive Certificate of Analysis (COA) detailing spectral properties (absorption/emission maxima, extinction coefficient), functional group activity, and purity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This material is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Blue to purple solid or lyophilized powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 90% (Dye content)
Absorption Maximum (λmax)	~346 nm
Emission Maximum (λem)	~442 nm
Extinction Coefficient (ε)	~19,000 cm⁻¹M⁻¹
Solubility	Soluble in water, DMSO, DMF
Functional Group	Succinimidyl Ester (NHS Ester), reactive towards amines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.