Description

5(6)-Aminofluorescein is a fluorescent dye derivative of fluorescein, characterized by the introduction of an amino group, which significantly alters its spectral and chemical properties. This modification makes it a highly valuable intermediate and active labeling reagent in the development of diagnostic assays and biochemical probes. It is primarily sought after by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for creating sensitive detection systems.

Application

• Fluorescent Labeling Reagent: Used for tagging proteins, antibodies, and nucleic acids in immunoassays (e.g., ELISA, Western blot) and fluorescence in situ hybridization (FISH).
• Biochemical Probe Synthesis: Serves as a key precursor for synthesizing more complex fluorescent probes and sensors for detecting enzymes, ions, or small molecules.
• Diagnostic Kit Manufacturing: Incorporated into lateral flow assays and other point-of-care diagnostic devices as a signal-generating component.
• Cell Staining and Microscopy: Employed in histological and cytological studies for visualizing cellular structures and processes.
• Material Science Research: Used in the development of fluorescent polymers and nanoparticles for sensing and imaging applications.
• HPLC and CE Detection: Acts as a derivatization agent to enhance the detection sensitivity of amines, amino acids, and other analytes in chromatographic separations.

Basic Information

Product Name	5(6)-Aminofluorescein
CAS No.	27599-63-9
Molecular Formula	C20H13NO5
Molecular Weight	347.32 g/mol
Synonyms	5-Aminofluorescein; 6-Aminofluorescein; Aminofluorescein Isomer Mixture; 3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(or 6)-amine; Fluoresceinamine Isomer I & II; 5(6)-AF
EINECS	Contact for details

Quality Control

Our 5(6)-Aminofluorescein is produced under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, spectral data, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment away from direct light exposure to maintain stability and performance.

Specification

Item	Specification
Appearance	Orange to red-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (sum of isomers)
Loss on Drying	≤ 2.0 %
Fluorescence	Conforms (Ex/Em ~490/515 nm in buffer)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.