Description

5-Nitrofluorescein is a nitro-substituted derivative of fluorescein, a key synthetic intermediate and analytical reagent. This compound is valued for its role in the synthesis of more complex fluorescent dyes and as a versatile building block in organic chemistry. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent sectors.

Application

• Fluorescent Dye Synthesis: A crucial precursor for manufacturing advanced fluorescent probes and tags used in bioimaging and flow cytometry.
• Bioconjugation & Labeling: Used to create labeled antibodies, proteins, and nucleotides for various detection and diagnostic assays.
• Analytical Chemistry: Serves as a standard or reagent in spectrophotometric and fluorometric analysis methods.
• Research & Development: Employed in academic and industrial R&D for studying photophysical properties and developing new sensor materials.
• pH Indicator Development: Acts as a starting material for synthesizing novel pH-sensitive dyes.

Basic Information

Product Name	5-Nitrofluorescein
CAS No.	27402-68-2
Molecular Formula	C20H11NO7
Molecular Weight	377.30 g/mol
Synonyms	5-Nitrofluorescein; 3',6'-Dihydroxy-5-nitro-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 5-Nitro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; Fluorescein, 5-nitro-; 5-Nitrofluorane; 5-NF
EINECS	Contact for details

Quality Control

Our 5-Nitrofluorescein is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A Certificate of Analysis (COA) detailing all specifications is provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C) under an inert atmosphere to prevent oxidation. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.