Description

Fluorescein Isothiocyanate (FITC) is a vital fluorescent labeling reagent widely used in biochemistry and cell biology. Its core value lies in its ability to form stable conjugates with proteins and antibodies, enabling high-sensitivity detection and imaging. This compound is essential for researchers and manufacturers in the biotechnology, diagnostics, and pharmaceutical industries who require reliable fluorescent probes for applications like flow cytometry, immunofluorescence, and fluorescence microscopy.

Application

• Fluorescent Antibody Labeling: Conjugation to antibodies for use in immunofluorescence assays (IFA) and immunohistochemistry (IHC).
• Flow Cytometry: Cell surface and intracellular protein staining for analysis and sorting of cell populations.
• Fluorescence Microscopy: Tagging of biomolecules to visualize localization and dynamics within cells and tissues.
• Diagnostic Kit Manufacturing: A key component in the production of fluorescent lateral flow assays and other diagnostic tools.
• Protein & Peptide Tracing: Labeling of proteins to study their purification, binding kinetics, and degradation.
• Nucleic Acid Labeling: Indirect labeling of oligonucleotides for fluorescence in situ hybridization (FISH) techniques.

Basic Information

Product Name	Fluorescein Isothiocyanate
CAS No.	27072-45-3
Molecular Formula	C21H11NO5S
Molecular Weight	389.38 g/mol
Synonyms	FITC; Fluorescein 5-isothiocyanate; 5-FITC; 5-Isothiocyanatofluorescein; Isomer I of Fluorescein Isothiocyanate; Fluorescein Isothiocyanate Isomer I; 3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5'-isothiocyanate
EINECS	Contact for details

Quality Control

Our Fluorescein Isothiocyanate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive; prolonged exposure to moisture or light will degrade performance. Keep the container tightly sealed in a desiccated environment after each use.

Specification

Item	Specification
Appearance	Orange to brownish-orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90% (Isomer I)
Absorption Maximum	~495 nm
Emission Maximum	~519 nm
Fluorescence Intensity	Conforms to reference standard
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.