Description

Methyl Calcein CAS NO 25639-40-1 is a fluorescent dye and calcium indicator widely used in biological research. Its core value lies in its high sensitivity and selectivity for calcium ions, enabling real-time visualization and quantification of intracellular calcium dynamics. This compound is essential for researchers and professionals in life sciences, particularly for applications in cell biology, neuroscience, and high-throughput screening.

Application

• Intracellular Calcium Imaging: Primary use as a fluorescent probe for visualizing calcium ion (Ca²⁺) flux in live cells using fluorescence microscopy.
• Neuroscience Research: Monitoring neuronal activity and synaptic transmission by detecting changes in cytoplasmic calcium concentrations.
• Cell Viability Assays: Employed in assays to measure cell health, apoptosis, and membrane integrity based on calcium-dependent fluorescence.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify compounds that modulate calcium signaling pathways.
• Enzymology Studies: Acting as a substrate for esterases; the non-fluorescent acetoxymethyl ester form (Calcein AM) is cleaved intracellularly to yield the fluorescent Methyl Calcein.
• Fluorescence-Activated Cell Sorting (FACS): Used as a fluorescent marker for analyzing and sorting cell populations based on calcium levels.

Basic Information

Item	Details
Product Name	Methyl Calcein
CAS No.	25639-40-1
Molecular Formula	C31H26N2O13
Molecular Weight	634.55 g/mol
Synonyms	Calcein Methyl Ester; NSC 113992; Fluorexon Methyl Ester; 2,7-Bis[bis(carboxymethyl)amino]methylfluorescein Methyl Ester; 2',7'-Bis-(bis-carboxymethyl-aminomethyl)-fluorescein Methyl Ester; Methyl Fluorexon; Methyl Derivative of Calcein
EINECS	Contact for details

Quality Control

Our Methyl Calcein is produced under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	Orange to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Fluorescence Properties	Ex/Em ~495/515 nm (Ca²⁺ bound)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.