Description

p-Nitrotetrazolium Violet is a specialized tetrazolium salt widely used as a vital staining agent and redox indicator in biochemical and histochemical applications. Its primary value lies in its ability to form a highly colored, water-insoluble formazan dye upon reduction, making it an essential tool for visualizing cellular dehydrogenase activity and cell viability. This compound is critically needed by researchers and quality control laboratories in the biotechnology, pharmaceutical, and life sciences sectors for assays, cytotoxicity testing, and metabolic studies.

Application

• Cell Viability and Cytotoxicity Assays: Used to quantify metabolically active cells, commonly in MTT assay alternatives for drug screening.
• Histochemical Staining: Employed as a marker for dehydrogenase enzyme activity in tissue sections for diagnostic and research purposes.
• Microbiological Testing: Acts as an indicator in microbial identification and metabolic activity tests.
• Biochemical Research: Serves as an electron acceptor in studies of mitochondrial function and respiratory chain activity.
• Quality Control in Cell Culture: Utilized for monitoring contamination and assessing the health of cell lines in biomanufacturing.
• Photographic and Imaging Reagents: Can be used in specialized photographic processes and as a component in imaging kits.

Basic Information

Product Name	p-Nitrotetrazolium Violet
CAS No.	25413-85-8
Molecular Formula	C₁₉H₁₅N₉O₄
Molecular Weight	433.39 g/mol
Synonyms	p-Nitro-NT; Nitrotetrazolium Violet; 2,2',5,5'-Tetraphenyl-3,3'-(p-nitrophenyl)ditetrazolium chloride; Tetrazolium Violet; NTV; p-Nitrotetrazolium Blue (historical); INT-V; 3,3'-(4-Nitrophenyl)bis(2,5-diphenyl-2H-tetrazol-3-ium) dichloride
EINECS	Contact for details

Quality Control

Our p-Nitrotetrazolium Violet is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and UV-Vis spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. A Certificate of Analysis (COA) detailing purity, absorbance characteristics, and performance in standard assays is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to reference spectrum
Identification (UV-Vis)	λmax conforms
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Absorbance Ratio (Amax/Amin)	≥ 3.0 (in suitable solvent)
Performance in Standard Assay	Forms formazan precipitate with characteristic color

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.