Description

Fluoresceinisothiocyanate (FITC) is a vital fluorescent labeling reagent widely used in biochemistry and molecular biology. Its core value lies in its ability to form stable conjugates with proteins and antibodies, enabling high-sensitivity detection and visualization in complex biological systems. This product is essential for researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors who require reliable, high-purity reagents for fluorescence-activated cell sorting (FACS), immunofluorescence (IF), and immunohistochemistry (IHC).

Application

• Antibody and Protein Labeling: Primary application for creating fluorescent conjugates used in immunoassays and flow cytometry.
• Cellular Imaging & Microscopy: Used in fluorescence microscopy to label and track cellular components, including cytoskeletal proteins.
• Flow Cytometry (FACS): A standard fluorophore for cell surface marker analysis and immunophenotyping.
• Diagnostic Kit Manufacturing: Key component in the production of fluorescent lateral flow assays and other in-vitro diagnostic (IVD) devices.
• Nucleic Acid Labeling: Can be used to label oligonucleotides for fluorescence in situ hybridization (FISH) applications.
• Bioconjugation & Probe Development: Serves as a starting material for synthesizing more complex fluorescent probes and sensors.

Basic Information

Product Name	Fluoresceinisothiocyanate
CAS No.	25168-13-2
Molecular Formula	C21H11NO5S
Molecular Weight	389.38 g/mol
Synonyms	FITC; Fluorescein 5-isothiocyanate; Fluorescein isothiocyanate isomer I; 5-FITC; 5-Isothiocyanatofluorescein; 3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5'-isothiocyanate; Isomer I of Fluorescein Isothiocyanate
EINECS	246-668-4

Quality Control

Our Fluoresceinisothiocyanate is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, fluorescence performance, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light will degrade performance. For long-term stability, store desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	Orange-red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90% (Isomer I)
Fluorescence	Bright green emission under blue light
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.