Description

Z-Nle-Onp is a specialized amino acid derivative, specifically the p-nitrophenyl ester of N-benzyloxycarbonyl-norleucine. This compound serves as a crucial protected building block in solid-phase peptide synthesis (SPPS), enabling the controlled elongation of peptide chains. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel peptides, peptidomimetics, and bioactive compounds, where precise coupling and high purity are essential for successful outcomes.

Application

• Solid-Phase Peptide Synthesis (SPPS): As an activated ester, it is used for the efficient incorporation of norleucine (Nle) into peptide sequences under mild conditions.
• Peptide Library Construction: A key reagent for generating diverse peptide libraries for high-throughput screening in drug discovery.
• Bioconjugation: Useful for the site-specific modification of peptides, proteins, or other biomolecules via amine-reactive chemistry.
• Research & Development: Employed in academic and industrial R&D labs for the synthesis of custom peptides, reference standards, and investigative probes.
• Pharmaceutical Intermediates: Acts as a critical intermediate in the multi-step synthesis of complex peptide-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	Z-Nle-Onp
CAS No.	24181-97-3
Molecular Formula	C₂₀H₂₂N₂O₆
Molecular Weight	386.40 g/mol
Synonyms	N-Cbz-Norleucine p-Nitrophenyl Ester; Nα-Benzyloxycarbonyl-L-norleucine 4-nitrophenyl ester; Z-L-Norleucine ONp ester; Cbz-Nle-ONp; N-Carbobenzoxy-L-norleucine p-nitrophenyl ester; (S)-4-(((Benzyloxy)carbonyl)amino)-6-(4-nitrophenoxy)-6-oxohexanoic acid; Benzyl N-[(2S)-1-[(4-nitrophenoxy)-oxo]-5-carboxypentyl]carbamate
EINECS	Contact for details

Quality Control

Our Z-Nle-Onp is manufactured under strict quality protocols to ensure batch-to-batch consistency and high chemical purity suitable for sensitive synthetic applications. Each lot undergoes comprehensive analytical testing, including HPLC for assay and purity, chiral analysis to confirm enantiomeric integrity, and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Purity (HPLC)	≥97.0%
Optical Rotation [α]²⁰D	Contact for details
Melting Point	Contact for details
Heavy Metals	<20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.