Description

Hoechst 33258 Analog 5 is a high-purity, synthetic organic compound widely recognized as a vital fluorescent dye and nucleic acid stain. This product is essential for researchers and industrial laboratories requiring precise, reliable tools for DNA quantification and cytogenetic analysis. It is primarily utilized in life science research, molecular biology, and diagnostic assay development for its selective binding properties.

Application

• Nucleic Acid Staining & Quantification: Used in fluorometric assays for the detection and quantification of double-stranded DNA (dsDNA) in solution.
• Cell Biology & Cytogenetics: Employed as a fluorescent stain for chromosome banding and visualization of nuclear DNA in fixed cells.
• Biochemical Research: Serves as a key reagent in studies investigating DNA-protein interactions and chromatin structure.
• Diagnostic Kit Manufacturing: Incorporated as a critical component in commercial kits for DNA content analysis and cell cycle studies.
• Flow Cytometry: Utilized for DNA content analysis and ploidy determination in cell populations.
• Microscopy & Imaging: Applied in fluorescence microscopy for detailed nuclear and chromosomal imaging.

Basic Information

Product Name	Hoechst 33258 Analog 5
CAS No.	23491-55-6
Molecular Formula	C25H24N6O
Molecular Weight	424.50 g/mol
Synonyms	Pibenzimol; Hoechst 33258 Derivative; Bisbenzimide H 33258 Analog; 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole; HOE 33258 Analog; DNA Stain 33258 Analog; Fluorescent DNA Probe Analog
EINECS	Contact for details

Quality Control

Our Hoechst 33258 Analog 5 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic identification. A Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive) and should be kept under anhydrous conditions. For long-term storage, consider desiccants or an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Fluorescence Properties	Conforms to reference
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.