Description

Hoechst 33258 Analog 2 is a high-purity, synthetic bis-benzimide derivative widely used as a fluorescent nucleic acid stain. This compound is valued for its strong binding affinity to adenine-thymine (A-T) rich regions of double-stranded DNA, making it a critical tool for molecular biology research and diagnostics. It is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and life science sectors requiring precise DNA quantification and visualization.

Application

• Fluorescent DNA Staining in gel electrophoresis for visualization of nucleic acids.
• Cell Biology Research for nuclear and chromosome staining in fluorescence microscopy.
• Flow Cytometry applications for DNA content and cell cycle analysis.
• Biomedical Diagnostics as a component in kits for detecting specific DNA sequences.
• Biochemical Research for studying DNA-protein interactions and chromatin structure.
• Quality Control in pharmaceutical manufacturing for nucleic acid contaminant testing.

Basic Information

Product Name	Hoechst 33258 Analog 2
CAS No.	23491-54-5
Molecular Formula	C25H24N6O · 3HCl · 3H2O
Molecular Weight	624.90 g/mol (as trihydrochloride trihydrate)
Synonyms	Pibenzimol; 2'-[4-Hydroxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bibenzimidazole trihydrochloride trihydrate; Hoechst 33258 Derivative; Bisbenzimide H 33258 analog; NSC 322921; DNA Stain 33258 Analog; A-T specific DNA minor groove binder
EINECS	Contact for details

Quality Control

Our Hoechst 33258 Analog 2 is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant quality guidelines suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	8.0% - 12.0%
Absorbance Ratio (A260/A340)	≤ 0.10
Fluorescence Enhancement	Conforms to performance standard
Solubility	Freely soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.