Description

Dimethylmethylene Blue Hydrochloride is a cationic phenothiazine dye widely recognized for its metachromatic staining properties. This compound is essential for the specific detection and quantification of sulfated glycosaminoglycans (GAGs), particularly in biochemical and diagnostic research. It is a critical reagent for laboratories and manufacturers in the pharmaceutical, biotechnology, and diagnostic kit production sectors, enabling precise analysis of connective tissue disorders and other metabolic conditions.

Application

• Biochemical Assays: Primary reagent for the quantitative colorimetric assay of sulfated glycosaminoglycans (GAGs) like chondroitin sulfate and dermatan sulfate.
• Diagnostic Research: Used in the development and manufacturing of diagnostic kits for mucopolysaccharidoses (MPS) such as Hunter and Sanfilippo syndromes.
• Histology & Cytology: Employed as a specific metachromatic stain for tissue sections to visualize mast cell granules and cartilage proteoglycans.
• Pharmaceutical R&D: Utilized in drug discovery and quality control processes to study drug effects on GAG metabolism and cartilage health.
• Biomaterial Analysis: Applied in the characterization of hydrogels and engineered tissues to assess sulfated GAG content.
• Veterinary Diagnostics: Adapted for use in veterinary medicine to diagnose similar metabolic storage diseases in animals.

Basic Information

Product Name	Dimethylmethylene Blue Hydrochloride
CAS No.	23481-50-7
Molecular Formula	C16H22ClN3S
Molecular Weight	323.88 g/mol
Synonyms	DMMB; 1,9-Dimethylmethylene Blue Zinc Chloride Double Salt; Dimethyl Methylene Blue; Basic Blue 22; C.I. 52040; Azure B Eosinate; 3,7-Bis(dimethylamino)phenothiazin-5-ium Chloride; Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride
EINECS	Contact for details

Quality Control

Our Dimethylmethylene Blue Hydrochloride is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with relevant standards for biochemical use. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and assay results is provided with every shipment to guarantee traceability and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	Dark green to blue-black powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
UV-Vis Absorption	Conforms (λmax ~ 640-660 nm)
Loss on Drying	≤ 5.0 %
Residue on Ignition	≤ 1.0 %
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.