Description

Tetrazole Blue Diformazan is a specialized chromogenic reagent and formazan dye widely utilized in biochemical and analytical applications. Its primary value lies in its ability to produce a distinct, measurable color change, making it an essential tool for quantitative assays and diagnostic tests. This compound is particularly critical for researchers and manufacturers in the life sciences, clinical diagnostics, and pharmaceutical development sectors who require reliable and sensitive detection methods.

Application

• Cell Viability and Cytotoxicity Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Diagnostic Test Kits: Serves as a chromogenic indicator in point-of-care and laboratory diagnostic kits for various biomarkers.
• Enzyme-Linked Immunosorbent Assay (ELISA): Acts as a detection reagent in colorimetric ELISA protocols for quantifying antigens or antibodies.
• Biochemical Research: Employed in research laboratories for studying enzyme kinetics, particularly dehydrogenase activity.
• Microbiology: Applied in tests for microbial growth and metabolic activity assessment.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to evaluate the effects of drug candidates on cell metabolism.

Basic Information

Product Name	Tetrazole Blue Diformazan
CAS No.	23305-71-7
Molecular Formula	C₁₈H₁₂N₁₀
Molecular Weight	392.36 g/mol
Synonyms	MTT Formazan; Thiazolyl Blue Tetrazolium Bromide Formazan; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide Formazan; MTT Diformazan; Tetrazolium Blue Formazan; MTT Reduction Product; Thiazolyl Blue Formazan; MTT Tetrazolium Formazan
EINECS	Contact for details

Quality Control

Our Tetrazole Blue Diformazan is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and reliability for sensitive research and diagnostic applications. Each lot undergoes comprehensive analysis, including purity assessment by HPLC and spectroscopic identification. A Certificate of Analysis (COA) detailing all quality parameters is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Dark blue to purple crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Absorbance Maximum	~570 nm (in solution)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.