Description

Methyl Green CAS NO 22383-16-0 is a synthetic cationic dye belonging to the triarylmethane class, widely recognized for its distinct green color and metachromatic properties. It serves as a critical biological stain for histology and cytology, enabling clear visualization of cellular structures like chromatin and nuclei under a microscope. This high-purity dye is essential for research and diagnostic laboratories in the life sciences, pharmaceutical development, and educational sectors, where consistent staining performance is paramount.

Application

• Histological Staining: Primary application as a nuclear stain in histology, often used in combination with other dyes for tissue differentiation.
• Cytological Analysis: Staining of chromatin and nuclei in cell smears and preparations for microscopic examination.
• Microbiological Staining: Used in specific staining protocols for bacteria and other microorganisms.
• Electrophoresis Marker: Serves as a colored tracking dye in gel electrophoresis procedures.
• Educational & Research: A standard reagent in university and research laboratory settings for teaching and experimental protocols.
• Indicator Applications: Potential use as a pH or redox indicator in certain analytical chemistry contexts.

Basic Information

Product Name	Methyl Green
CAS No.	22383-16-0
Molecular Formula	C26H33Cl2N3
Molecular Weight	458.47 g/mol
Synonyms	Basic Green 5; C.I. 42585; Ethyl Green; Methyl Green O; Methyl Green (C.I. 42585); Methyl Green zinc chloride salt; [4-[[4-(Dimethylamino)phenyl][4-(methylamino)-1-naphthalenyl]methylene]-2,5-cyclohexadien-1-ylidene]dimethylammonium chloride zinc chloride salt; Methyl Green zinc chloride double salt
EINECS	244-949-1

Quality Control

Our Methyl Green is manufactured under a rigorous quality management system. Each batch is tested to ensure it meets high-purity standards suitable for biological staining and research applications. Key parameters including identity, purity (assay), and heavy metal content are verified. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	Dark green powder or crystals with metallic luster
Identification (IR)	Conforms to structure
Assay (Dye Content)	≥ 85%
Loss on Drying	≤ 5.0%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in water (green solution), sparingly soluble in ethanol
Absorption Maximum	~630-635 nm (in water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.