Description

Neo-Tetrazolium, Diformazan CAS NO 21520-87-6 is a specialized formazan dye derivative, a critical chromogenic product formed in vital staining and enzymatic assays. This compound matters for its role as a colored, insoluble endpoint in biochemical reactions, particularly those involving dehydrogenase enzymes and cellular viability tests. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical R&D departments developing cell-based assays and histochemical staining protocols.

Application

• Key chromogen in MTT assay and related tetrazolium-based cell proliferation/viability tests.
• Histochemical staining agent for localizing dehydrogenase enzyme activity in tissue sections.
• Critical component in diagnostic kits for measuring microbial viability and metabolic activity.
• Research reagent in biochemical oxidation-reduction studies and electron transfer mechanisms.
• Used in the development of biosensors and colorimetric detection systems.
• Substrate in enzymatic assays for pharmaceutical screening and toxicology studies.

Basic Information

Product Name	Neo-Tetrazolium, Diformazan
CAS No.	21520-87-6
Molecular Formula	C₁₈H₂₀N₈O₂
Molecular Weight	380.41 g/mol
Synonyms	Neotetrazolium Diformazan; Diformazan, Neo-Tetrazolium; 2,2',5,5'-Tetraphenyl-3,3'-(p-diphenylene)ditetrazolium Diformazan; NT Diformazan; Neotetrazolium Formazan; Tetranitro Blue Tetrazolium Diformazan (related); TNBT Diformazan; Vital Staining Dye Diformazan
EINECS	Contact for details

Quality Control

Our Neo-Tetrazolium, Diformazan is produced and tested under a rigorous quality management system. Each batch undergoes comprehensive analysis to ensure identity, purity, and performance suitability for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity by HPLC, spectral identification, and relevant biological performance tests are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is light-sensitive and easily oxidized; for long-term storage, consider under an inert atmosphere. Keep container tightly sealed to minimize exposure to air and moisture.

Specification

Item	Specification
Appearance	Dark blue to violet crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMSO, DMF; insoluble in water
Biological Activity	Performance tested in standard MTT assay system

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.