Description

Dimidium is a vital cationic phenanthridine dye widely utilized in biochemical and analytical research. Its primary commercial value lies in its exceptional ability to selectively bind to nucleic acids, making it a critical reagent for vital staining and fluorescence-based assays. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries, particularly for applications in cell biology and molecular probe development. The compound is supplied as Dimidium CAS NO 20566-69-2 to ensure precise identification and sourcing.

Application

• Fluorescent Nucleic Acid Staining: Used as a selective fluorescent stain for DNA and RNA in gel electrophoresis and microscopy.
• Cell Viability Assays: Employed in combination with other dyes (e.g., ethidium homodimer) for live/dead cell differentiation.
• Mitochondrial Staining: Applied in research to visualize mitochondria in live cells due to its selective accumulation.
• Molecular Biology Research: Serves as a tool for studying nucleic acid structure, function, and protein interactions.
• Diagnostic Reagent Formulation: A key component in the development of certain clinical diagnostic kits and research reagents.
• Veterinary and Biological Research: Used in parasitology for staining trypanosomes and other blood parasites.

Basic Information

Product Name	Dimidium
CAS No.	20566-69-2
Molecular Formula	C20H18N3Cl
Molecular Weight	335.83 g/mol
Synonyms	3,8-Diamino-5-methyl-6-phenylphenanthridinium chloride; Dimidium Bromide analogue (chloride salt); Phenanthridinium, 3,8-diamino-5-methyl-6-phenyl-, chloride; Dimidium chloride; 6-Phenyl-5-methyl-3,8-diaminophenanthridinium chloride; Trypan Red (related compound); Homidium (related compound)
EINECS	Contact for details

Quality Control

Our Dimidium is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing to ensure high purity and consistency, meeting the rigorous standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	Dark red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 1.0 %
Solubility	Soluble in water, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.