Description

Janus Green B for Microscopy is a vital cationic phenazine dye widely used as a supravital stain in biological research. Its primary value lies in its selective staining properties, particularly for mitochondria and nerve tissue, enabling critical cellular analysis. This high-purity grade is essential for researchers, diagnostic laboratories, and educational institutions requiring reliable and consistent staining performance for microscopy and histology applications.

Application

• Mitochondrial Staining: As a supravital stain for visualizing mitochondria in living cells, a key application in cell biology and toxicology studies.
• Neurological Research: Staining of nerve fibers and Nissl substance in neural tissue for histological examination.
• Microscopy & Histology: A standard dye in laboratory kits and protocols for preparing biological samples for light microscopy.
• Bacteriological Staining: Used in specific differential staining techniques to identify and classify bacterial specimens.
• Educational Demonstrations: Employed in academic settings to teach fundamental principles of cell structure and staining techniques.
• Textile Dyeing (Historical/Industrial): While primarily biological, it also finds use as a dye for silk, wool, and leather.

Basic Information

Product Name	Janus Green B for Microscopy
CAS No.	2869-83-2
Molecular Formula	C30H31ClN6
Molecular Weight	511.06 g/mol
Synonyms	Janus Green; Diazin Green S; Union Green B; Janus Green B; CI 11050; Basic Blue 18; Janus Green B Salt; 3-(Diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenylphenazinium chloride
EINECS	220-695-6

Quality Control

Our Janus Green B for Microscopy is manufactured and tested to meet the stringent requirements of research and diagnostic applications. Each batch undergoes rigorous analytical testing, including spectrophotometric purity analysis and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing assay, absorbance characteristics, and solubility is provided to ensure traceability and performance consistency for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature between 15-25°C (59-77°F) in a dry, well-ventilated area. Keep the container tightly sealed when not in use to protect against moisture and contamination.

Specification

Item	Specification
Appearance	Dark green to greenish-black powder
Identification (IR)	Conforms to structure
Assay (Dye Content)	≥ 85% (by spectrophotometry)
Absorbance Maximum (λmax)	~615 nm (in ethanol)
Solubility	Soluble in water and ethanol
Loss on Drying	≤ 5.0 %
Ash Content	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.