Description

Janus Green B is a synthetic, cationic phenazine dye widely used as a vital biological stain and redox indicator. Its unique electrochemical properties make it a critical tool for assessing mitochondrial activity and cellular respiration in life science research. This high-purity compound is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic sectors requiring reliable, consistent staining performance.

Application

• Biological Staining: Vital staining for mitochondria in live cell microscopy and histology to assess cell viability and metabolic state.
• Microbiological Research: Used as a redox indicator in bacterial culture media and for studying microbial respiration.
• Cytology & Histology: A key component in specialized staining kits for visualizing cellular structures, particularly in nerve tissue and blood cell analysis.
• Biochemical Assays: Serves as an electron acceptor in enzymatic assays and studies of oxidative phosphorylation.
• Textile Dyeing: Employed in laboratory-scale dyeing of silk, wool, and nylon for research purposes.
• Electrochemistry: Utilized as a mediator in biosensor development and electrochemical studies due to its reversible redox behavior.

Basic Information

Product Name	Janus Green B
CAS No.	2869-83-2
Molecular Formula	C30H31ClN6
Molecular Weight	511.06 g/mol
Synonyms	Janus Green; Diazin Green S; Union Green B; CI 11050; Basic Blue 18; 3-(Diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenylphenazinium chloride; Janus Green B Stain; Diazine Green S
EINECS	220-695-0

Quality Control

Our Janus Green B is manufactured under a strict quality management system to ensure batch-to-batch consistency suitable for sensitive biological applications. Each lot undergoes comprehensive analytical testing, including identity confirmation, purity assay, and impurity profiling. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Dark green to bronze-green powder or crystals
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Solubility	Soluble in water and ethanol
Absorption Maximum (λmax)	~660 nm (in water)
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.