Description

Xylene Cyanole Ff is a synthetic triphenylmethane dye widely used as a tracking dye in molecular biology and analytical chemistry. Its primary value lies in its distinct blue color and predictable electrophoretic mobility, which provides a crucial visual reference point during separation processes. This product is essential for research laboratories, diagnostic kit manufacturers, and quality control departments in the biopharmaceutical industry requiring precise gel and capillary electrophoresis.

Application

• As a tracking or marker dye in agarose and polyacrylamide gel electrophoresis (PAGE) for nucleic acid and protein analysis.
• Component in DNA loading buffers to monitor the progress of electrophoresis runs.
• Use in capillary electrophoresis applications as an internal mobility standard.
• Preparation of molecular weight standards and diagnostic reagents for life science research.
• As a colored indicator in certain biochemical assays and experimental protocols.
• Potential use as a histological stain in microscopy, though less common than other dyes.

Basic Information

Product Name	Xylene Cyanole Ff
CAS No.	2650-17-1
Molecular Formula	C25H27N2NaO6S2
Molecular Weight	538.61 g/mol
Synonyms	Acid Blue 147; C.I. 42135; Xylene Cyanol FF; Xylene Cyanol FCF; Cyanole; Acid Blue 147, sodium salt; Brilliant Acid Blue BS; 1,3-Benzenedisulfonic acid, 4,4'-(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azobis[6-(diethylamino)-, disodium salt
EINECS	220-181-8

Quality Control

Our Xylene Cyanole Ff is manufactured and tested to ensure high purity and batch-to-batch consistency suitable for sensitive analytical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as absorbance profile, dye content, and impurity levels. We adhere to rigorous in-house quality standards to meet the demands of research and industrial use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Keep away from incompatible materials. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark blue to blue-black powder
Identification (IR)	Conforms to structure
Assay (Dye Content)	≥ 85%
Absorbance Maximum (λmax)	610 - 630 nm
Loss on Drying	≤ 10.0 %
Solubility (in Water)	Clear, intense blue solution
Electrophoretic Performance	Meets functional test criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.