Description

Sulforhodamine B CAS NO 2609-88-3 is a high-purity, water-soluble xanthene dye widely recognized for its intense red fluorescence. This compound is a critical reagent in life science research and industrial applications due to its excellent photostability and high quantum yield. It is primarily needed by biotechnology companies, academic research institutions, and diagnostic manufacturers for applications in fluorescence microscopy, cell viability assays, and protein labeling.

Application

• Fluorescent Labeling and Staining: Used as a fluorescent tracer in biological research for labeling proteins, nucleic acids, and cellular structures in fluorescence microscopy and flow cytometry.
• Cell Viability and Cytotoxicity Assays (SRB Assay): The Sulforhodamine B (SRB) assay is a standard colorimetric method for measuring cell density and proliferation, commonly used in drug discovery and cancer research.
• Industrial Dye and Tracer: Employed as a colorant in various industrial processes and as a fluorescent water tracer in hydrological and environmental studies.
• Laser Dye: Suitable for use as a gain medium in tunable dye lasers due to its absorption and emission properties in the visible spectrum.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of fluorescent diagnostic kits and reagents.
• Material Science Research: Used in the development and testing of fluorescent polymers, sensors, and advanced materials.

Basic Information

Product Name	Sulforhodamine B
CAS No.	2609-88-3
Molecular Formula	C27H29N2NaO7S2
Molecular Weight	580.65 g/mol
Synonyms	Sulforhodamine B Sodium Salt; Acid Red 52; C.I. 45100; Lissamine Rhodamine B; Sulforhodamine B Extra; Xylene Red B; Kiton Red 620; Sulforhodamine B (SRB); 3',6'-Bis(diethylamino)-2-{[(2,4-disulfophenyl)methylidene]amino}spiro[2-benzofuran-3,9'-xanthene]-1-one sodium salt
EINECS	220-036-2

Quality Control

Our Sulforhodamine B is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and identity confirmation. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	Dark Red to Purple Powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 95.0%
Absorbance Maximum (λmax)	565 - 570 nm
Emission Maximum	585 - 590 nm
Loss on Drying	≤ 5.0 %
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (in Water)	Clear, Red Solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.