Description

Nile Blue Chloride is a cationic phenoxazine dye widely recognized for its role as a biological stain and fluorescent probe. Its value lies in its specific affinity for certain cellular components and its ability to function as a redox indicator, making it a critical tool for research and diagnostics. This compound is essential for professionals in life sciences, histology, and industrial quality control who require precise staining and detection methods.

Application

• Biological Staining: Used as a metachromatic stain for lipids, amyloid, and mast cells in histopathology and cytology.
• Fluorescent Microscopy: Acts as a fluorescent probe for visualizing acidic organelles like lysosomes and for detecting apoptosis.
• Redox Indicator: Serves as an indicator in biochemical assays to monitor oxidation-reduction reactions due to its distinct color change.
• Textile Dyeing: Employed in specialized textile applications and as a colorant for inks and other materials.
• Research Reagent: A key component in laboratory research for studying cell biology, neurology, and material science.
• Industrial Quality Control: Utilized in specific industrial processes for colorimetric testing and quality assurance protocols.

Basic Information

Product Name	Nile Blue Chloride
CAS No.	2381-85-3
Molecular Formula	C₂₀H₂₀ClN₃O
Molecular Weight	353.85 g/mol
Synonyms	Nile Blue A; Nile Blue Chloride; Nile Blue 2B; C.I. 51180; Basic Blue 12; 5-Amino-9-(diethylamino)benzo[a]phenoxazin-7-ylium chloride; Nile Blue Perchlorate (related salt)
EINECS	219-178-5

Quality Control

Our Nile Blue Chloride is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and reliability for critical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We adhere to relevant industry standards to support research, diagnostic, and industrial requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Dark green powder or crystals with bronze luster
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Solubility	Soluble in water and ethanol
Absorption Maxima	~635-640 nm (in ethanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.