Description

Fluorescein is a highly versatile and intensely fluorescent synthetic organic compound belonging to the xanthene dye family. Its exceptional ability to absorb and emit light makes it a critical tracer and diagnostic agent across multiple high-value industries. This product is essential for manufacturers and researchers in the life sciences, medical diagnostics, and industrial monitoring sectors who require a reliable, high-purity fluorescent marker.

Application

• Ophthalmological Diagnostics: As a vital stain in fluorescein angiography to diagnose and monitor retinal disorders, diabetic retinopathy, and corneal abrasions.
• Biomedical Research & Staining: Used as a fluorescent tracer in flow cytometry, immunofluorescence, and cell viability assays (often conjugated to antibodies or other biomolecules).
• Industrial Leak Detection & Hydrology: Employed as a brilliant water-tracing dye to detect leaks in plumbing, HVAC systems, and to study water flow patterns in environmental science.
• Forensic Science: Utilized as a latent bloodstain detector (Luminol alternative) due to its reaction with hemoglobin, producing chemiluminescence.
• pH Indicator: Functions as a visual pH indicator, changing from yellow-green to orange-red in the pH range of approximately 4.5 to 6.5.
• Dye Manufacturing Intermediate: Serves as a key precursor for synthesizing derivatives like Fluorescein isothiocyanate (FITC), a cornerstone reagent for fluorescent labeling.

Basic Information

Product Name	Fluorescein
CAS No.	2321-07-5
Molecular Formula	C20H12O5
Molecular Weight	332.31 g/mol
Synonyms	Fluorescein; D&C Yellow No. 7; CI 45350; Solvent Yellow 94; Acid Yellow 73; Uranine (disodium salt); 9-(o-Carboxyphenyl)-6-hydroxy-3H-xanthen-3-one; 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; Resorcinolphthalein
EINECS	219-031-8

Quality Control

Our Fluorescein is produced under a strict quality management system to ensure batch-to-batch consistency and high performance. We offer grades suitable for industrial, technical, and research applications, with specifications tailored to meet customer requirements. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to guarantee traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Orange-red to dark red powder
Identification (IR)	Conforms to standard
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %
Fluorescence	Visual check conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.