Description

Z-Ile-Onp is a protected amino acid derivative, specifically the p-nitrophenyl ester of N-benzyloxycarbonyl-L-isoleucine, widely utilized as a crucial building block in peptide synthesis. Its primary value lies in providing a reactive carboxyl group for coupling reactions while the Z-group offers orthogonal protection for the amine, enabling the controlled construction of complex peptide sequences. This high-purity intermediate is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors developing novel therapeutic peptides, diagnostic agents, and biochemical tools.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key reagent for the efficient coupling of isoleucine residues in automated and manual peptide assembly.
• Solution-Phase Peptide Synthesis: Used in classical segment condensation strategies for producing longer or modified peptide chains.
• Pharmaceutical Intermediate: Serves as a critical raw material in the research and development of peptide-based active pharmaceutical ingredients (APIs).
• Bioconjugation: Facilitates the site-specific attachment of peptides to other molecules, such as proteins, polymers, or surfaces, for creating targeted drug delivery systems or diagnostic probes.
• Biochemical Research: Employed in academic and industrial labs to synthesize custom peptides for studying protein-protein interactions, enzyme substrates, or receptor ligands.
• Production of Protected Dipeptides and Tripeptides: Acts as a starting material for generating more complex, partially protected peptide fragments.

Basic Information

Product Name	Z-Ile-Onp
CAS No.	2130-99-6
Molecular Formula	C₂₀H₂₂N₂O₆
Molecular Weight	386.40 g/mol
Synonyms	N-Cbz-L-Isoleucine p-Nitrophenyl Ester; Nα-Benzyloxycarbonyl-L-isoleucine 4-nitrophenyl ester; Z-L-Ile-ONp; Cbz-Ile-ONp; (S)-2-(((Benzyloxy)carbonyl)amino)-3-methylpentanoic acid 4-nitrophenyl ester; N-Carbobenzoxy-L-isoleucine p-nitrophenyl ester; Benzyl N-[(2S,3S)-1-hydroxy-3-methyl-1-oxopentan-2-yl]carbamate 4-nitrophenyl ester analog
EINECS	218-365-6

Quality Control

Our Z-Ile-Onp CAS NO 2130-99-6 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis for enantiomeric excess, and identity confirmation by IR and NMR spectroscopy. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or cGMP guidelines for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant to maintain stability and prevent hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Melting Point	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents	Complies with ICH guidelines
Water Content (KF)	< 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.