Description

2,3,5-Triphenyltetrazolium Chloride is a vital organic salt widely recognized as a key redox indicator and biochemical reagent. Its primary value lies in its ability to form a vivid, water-insoluble red formazan dye upon reduction, serving as a critical marker for cellular viability and enzymatic activity. This compound is essential for researchers and quality control professionals in the pharmaceutical, biotechnology, and agricultural science sectors, where precise detection of metabolic processes is paramount.

Application

• Microbiological and Cell Culture Viability Assays (MTT/TTC Assays): Used as TTC for the colorimetric determination of living cells and bacterial colonies.
• Seed Viability Testing: A standard reagent in agricultural and botanical research to assess seed germination potential.
• Enzymatic Activity Detection: Employed as an electron acceptor in dehydrogenase enzyme assays, including those for succinate dehydrogenase.
• Histochemical Staining: Utilized in tissue sections to localize sites of metabolic activity and ischemia (e.g., in myocardial infarction research).
• Pharmaceutical Quality Control: Acts as a reagent in sterility testing and antimicrobial effectiveness testing.
• Biochemical Research: Serves as a probe in studies of mitochondrial function and respiratory chain activity.
• Food Safety Testing: Applied in rapid microbiological methods to detect microbial contamination.

Basic Information

Product Name	2,3,5-Triphenyltetrazolium Chloride
CAS No.	298-96-4
Molecular Formula	C19H15ClN4
Molecular Weight	334.80 g/mol
Synonyms	TTC; Triphenyltetrazolium Chloride; Tetrazolium Red; Red Tetrazolium; 2,3,5-Triphenyl-2H-tetrazolium Chloride; TPTZ; TZ; NSC 1771
EINECS	206-071-2

Quality Control

Our 2,3,5-Triphenyltetrazolium Chloride is manufactured under a strict quality management system. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for critical laboratory applications. Certificates of Analysis (COA) detailing identity, purity, and related substances are provided to guarantee traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, colorless solution in ethanol
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.