Description

Neotetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical and histochemical staining. Its primary value lies in its ability to form a highly colored, insoluble formazan dye upon reduction, making it indispensable for visualizing cellular metabolic activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries, particularly for assays like MTT and other cell viability tests.

Application

• Cell Viability and Proliferation Assays: Key component in the MTT assay and related tetrazolium reduction tests to measure mitochondrial activity in live cells.
• Histochemistry and Cytochemistry: Used as a chromogenic agent to localize dehydrogenase enzyme activity in tissue sections and cell cultures.
• Microbiology: Employed in tests to assess microbial viability and metabolic function.
• Biochemical Research: Serves as an electron acceptor in numerous enzymatic studies, particularly for oxidoreductases.
• Diagnostic Kits: Incorporated into commercial test kits for clinical and research purposes to detect specific metabolic pathways.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to evaluate the cytotoxic or cytoprotective effects of drug candidates.

Basic Information

Product Name	Neotetrazolium Chloride
CAS No.	298-95-3
Molecular Formula	C40H32Cl2N8
Molecular Weight	695.64 g/mol
Synonyms	2,2'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(3,5-diphenyl-2H-tetrazol-3-ium) dichloride; NT; Neotetrazolium Blue Chloride; NT Chloride; Tetrazolium Blue Chloride; Diazanylidene; Neotetrazolium dichloride
EINECS	206-068-6

Quality Control

Our Neotetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to greenish-yellow powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in water and ethanol
Biological Staining Test	Passes performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.