Description

Nitrotetrazolium Blue Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical assays. Its primary value lies in its ability to form a deep blue, water-insoluble formazan dye upon reduction, making it an essential tool for detecting cellular dehydrogenase activity and superoxide production. This compound is critically needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors for applications such as cell viability testing and histochemical staining.

Application

• Cell Viability and Cytotoxicity Assays: Used as a substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Histochemistry and Cytochemistry: Employed as a chromogen to localize dehydrogenase enzyme activity in tissue sections and cell smears.
• Superoxide Anion Detection: Acts as a sensitive indicator for the detection of superoxide radicals generated in biochemical systems, such as in neutrophil function tests.
• Microbiology: Utilized in tests for bacterial identification and metabolic activity studies.
• Biochemical Research: Serves as an electron acceptor in numerous enzymatic assays to study redox reactions.
• Diagnostic Kit Manufacturing: Incorporated as a key reagent in commercial diagnostic kits for clinical and research purposes.

Basic Information

Product Name	Nitrotetrazolium Blue Chloride
CAS No.	298-83-9
Molecular Formula	C40H30Cl2N10O6
Molecular Weight	817.64 g/mol
Synonyms	NBT; Nitro Blue Tetrazolium Chloride; 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium Chloride; Tetranitro Blue Tetrazolium Chloride; NBT Chloride; Nitrotetrazolium Blue; p-Nitro Blue Tetrazolium Chloride; NTB
EINECS	206-074-5

Quality Control

Our Nitrotetrazolium Blue Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with relevant specifications for research and diagnostic use. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to guarantee traceability and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to yellow-brown powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.