Description

Fluorescein is a highly versatile and intensely fluorescent synthetic organic compound belonging to the xanthene dye family. Its exceptional ability to absorb and emit light makes it a critical tracer and diagnostic agent across numerous scientific and industrial fields. This product is essential for professionals in the life sciences, medical diagnostics, and industrial process monitoring who require a reliable and high-purity fluorescent marker. Fluorescein CAS NO 232-07-5 is supplied with stringent quality control to ensure consistent performance in sensitive applications.

Application

• Ophthalmology & Medical Diagnostics: As a vital stain in fluorescein angiography to diagnose retinal and corneal disorders, and in applanation tonometry.
• Life Science Research & Cell Biology: Widely used as a fluorescent tracer and tag (FITC derivative) for labeling antibodies, proteins, and nucleic acids in flow cytometry, immunofluorescence (IF), and fluorescence microscopy.
• Hydrology & Environmental Tracing: Employed as a non-toxic fluorescent dye to trace water flow, study groundwater movement, and detect leaks in plumbing and industrial systems.
• Industrial Process Control: Utilized as a fluorescent marker in antifreeze and coolant formulations for leak detection, and in various manufacturing processes for flow visualization.
• Biochemical Assays & Diagnostics: Serves as a substrate or indicator in enzymatic assays (e.g., β-galactosidase) and in pH-sensitive fluorescent probes.
• Forensic Science: Applied as a latent bloodstain enhancer, where it reacts with the heme group in blood to produce strong fluorescence under blue-green light.

Basic Information

Product Name	Fluorescein
CAS No.	232-07-5
Molecular Formula	C20H12O5
Molecular Weight	332.31 g/mol
Synonyms	Fluorescein; D&C Yellow No. 7; CI 45350; Solvent Yellow 94; Acid Yellow 73; Uranine; Resorcinolphthalein; 9-(o-Carboxyphenyl)-6-hydroxy-3H-xanthen-3-one; 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
EINECS	205-438-8

Quality Control

Our Fluorescein is manufactured under a strict quality management system. Every batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for diagnostic and research applications. A detailed Certificate of Analysis (COA) documenting identity, purity, and related substances is provided with each shipment. We can supply material compliant with various pharmacopeial standards (e.g., USP, EP) upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture, which can affect performance.

Specification

Item	Specification
Appearance	Orange-red to red powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.2%
Fluorescein Isomers (HPLC)	Reported on COA
Heavy Metals (as Pb)	≤ 20 ppm
Fluorescence	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.