Description

Azido-PEG4-Boc-Hydrazide is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and a Boc-protected hydrazide. This compound is specifically engineered for bioorthogonal conjugation chemistry, enabling the precise and stable linkage of biomolecules under mild, aqueous conditions. It is an essential reagent for researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science, particularly for creating antibody-drug conjugates (ADCs), peptide modifications, and functionalized surfaces.

Application

• Bioconjugation and ADC (Antibody-Drug Conjugate) Development: The azide group enables copper-free click chemistry with alkyne-modified drugs or toxins, while the protected hydrazide allows for subsequent controlled coupling to carbonyl groups (e.g., oxidized glycans on antibodies).
• Peptide and Protein Modification: Used to introduce PEG spacers (PEGylation) to enhance solubility, stability, and pharmacokinetic properties of therapeutic peptides and proteins.
• Surface Functionalization and Material Science: Facilitates the creation of biofunctional coatings on nanoparticles, biosensors, and diagnostic arrays through sequential click and deprotection/coupling steps.
• Polymer and Dendrimer Synthesis: Serves as a versatile building block for constructing complex, branched polymeric architectures with defined chemical handles for further derivatization.
• Chemical Biology Probes: Enables the synthesis of activity-based probes and affinity tags for studying protein function and interactions in live cells.
• Diagnostic Reagent Preparation: Used in the development of immunoassay components and imaging agents that require stable, site-specific labeling.

Basic Information

Product Name	Azido-PEG4-Boc-Hydrazide
CAS No.	1919045-01-4
Molecular Formula	C18H35N5O7
Molecular Weight	433.5 g/mol
Synonyms	N-(Boc-hydrazinyl)-3,6,9,12-tetraoxapentadecan-15-azide; 15-Azido-4,7,10,13-tetraoxa-3-azahexadecan-1-oic acid tert-butyl hydrazide; Azido-PEG4-t-butyloxycarbonyl-hydrazide; Boc-NH-NH-PEG4-N3; 2-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethoxy]ethyl (2-{[(tert-butoxy)carbonyl]amino}ethyl)carbamate; tert-Butyl (2-{2-[2-(2-{2-[2-(2-azidoethoxy)ethoxy]ethoxy}ethoxy)ethoxy]ethyl}hydrazine-1-carboxylate
EINECS	Contact for details

Quality Control

Our Azido-PEG4-Boc-Hydrazide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.