Description

Trisulfo-Cy3-Alkyne is a functionalized cyanine dye derivative featuring a reactive alkyne group and three sulfonate moieties. This compound is engineered to provide exceptional water solubility and bioorthogonal reactivity, making it a critical tool for advanced labeling and detection applications. It is primarily utilized by researchers and developers in the fields of bioconjugation, bioimaging, and diagnostic assay development.

Application

• Bioorthogonal Labeling: Click chemistry conjugation with azide-modified biomolecules (proteins, antibodies, oligonucleotides) for fluorescent tagging.
• Live-Cell and In Vivo Imaging: Synthesis of near-infrared (NIR) fluorescent probes for deep-tissue imaging due to enhanced hydrophilicity and reduced nonspecific binding.
• Diagnostic Reagent Development: Conjugation to detection antibodies or oligonucleotides for use in fluorescence-based immunoassays (e.g., ELISA, lateral flow) and FISH probes.
• Flow Cytometry & Microscopy: Creation of highly soluble fluorescent antibody conjugates for cell surface marker staining and intracellular tracking.
• Material Science Functionalization: Covalent attachment to nanoparticles, polymers, or surfaces to impart fluorescence and biocompatibility.
• Proteomics & Biomarker Discovery: Labeling of proteins or peptides for gel-based or liquid chromatography analysis.

Basic Information

Product Name	Trisulfo-Cy3-Alkyne
CAS No.	1895849-34-9
Molecular Formula	C37H41N3O9S33- • 3X+ (X= counter ion)
Molecular Weight	Contact for details
Synonyms	Cy3 trisulfonate alkyne; Sulfo-Cyanine3 alkyne, trisodium salt; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-((1E,3E)-3-(1-(6-(prop-2-yn-1-yloxy)-6-oxohexyl)-3,3-dimethyl-5-sulfo-2,3-dihydro-1H-indol-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate; Trisulfonated Cy3 Alkyne; Water-soluble Cy3 alkyne
EINECS	Contact for details

Quality Control

Our Trisulfo-Cy3-Alkyne is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for optical property validation. Certificates of Analysis (COA) detailing batch-specific results are available upon request to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to purple solid or lyophilized powder
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption (λmax)	~550 nm
Emission (λmax)	~570 nm
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 5.0 %
Functional Group Integrity (Alkyne)	Conforms by assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.