Description

Cy5.5 Dbco is a near-infrared fluorescent dye functionalized with a dibenzocyclooctyne (DBCO) group for bioorthogonal click chemistry. This compound matters for researchers and developers requiring high-sensitivity, low-background labeling of biomolecules in complex biological systems without the need for cytotoxic metal catalysts. It is essential for professionals in bioconjugation, in vivo imaging, diagnostic assay development, and pharmaceutical research who need reliable, site-specific tagging.

Application

• Bioorthogonal Labeling: Site-specific conjugation to azide-modified antibodies, proteins, peptides, and oligonucleotides for creating fluorescent probes.
• In Vivo & In Vitro Imaging: Near-infrared fluorescence imaging for deep-tissue penetration in preclinical animal studies and live-cell imaging.
• Diagnostic Assay Development: Fluorescent tagging for lateral flow assays, ELISA, and other immunoassay platforms requiring high sensitivity.
• Drug Delivery & Targeting: Conjugation to drug carriers, nanoparticles, and targeting ligands for traceable delivery and biodistribution studies.
• Flow Cytometry & Microscopy: Cell surface labeling and intracellular tracking using fluorescence-activated cell sorting (FACS) and confocal microscopy.
• Biomaterial & Hydrogel Functionalization: Incorporation into polymers and scaffolds for traceable biomaterials in tissue engineering.

Basic Information

Product Name	Cy5.5 Dbco
CAS No.	1857352-95-4
Molecular Formula	C52H59N4O8S2+
Molecular Weight	923.18 g/mol
Synonyms	Sulfo-Cyanine5.5 DBCO; DBCO-Cy5.5; Cyanine5.5 Dibenzocyclooctyne; DBCO-Sulfo-Cy5.5; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-2-((E)-2-((E)-3-((E)-2-(1-(3-((3aS,4S,6aR)-2-(1,3-dioxoisoindolin-2-yl)hexahydro-1H-thieno[3,4-d]imidazol-4-yl)propyl)thioureido)vinyl)-5,5-dimethylcyclohex-2-en-1-ylidene)ethylidene)indolin-1-ium; DBCO-Cy5.5 SE Ester; Near-IR DBCO Dye
EINECS	Contact for details

Quality Control

Our Cy5.5 Dbco is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for dye performance validation. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to ensure compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~683 nm
Emission Maximum (λem)	~703 nm
Extinction Coefficient (ε)	≥ 190,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.