Description

Acid-PEG3-NHS Ester is a heterobifunctional crosslinking reagent featuring a carboxylic acid group and an amine-reactive N-hydroxysuccinimide (NHS) ester, connected by a triethylene glycol (PEG3) spacer. This compound is essential for creating stable amide bonds in aqueous buffers, enabling the precise conjugation of biomolecules such as peptides, proteins, and antibodies to various surfaces, particles, or other molecules. It is a critical tool for researchers and manufacturers in bioconjugation, diagnostic assay development, and targeted drug delivery systems, where controlled linker length and hydrophilicity are required.

Application

• Bioconjugation and Protein Labeling: Covalently attach dyes, drugs, or affinity tags to proteins and antibodies via amine groups.
• Diagnostic Reagent Synthesis: Manufacture enzyme-linked immunosorbent assay (ELISA) components and other immunoassay reagents.
• ADC (Antibody-Drug Conjugate) Linker: Serve as a hydrophilic, cleavable spacer in the construction of next-generation ADCs.
• Surface Functionalization: Modify nanoparticles, microspheres, biosensor chips, and other solid supports with carboxylic acid groups for further coupling.
• PEGylation Reagent: Introduce a short, hydrophilic PEG chain to biomolecules to improve solubility and stability.
• Peptide Synthesis: Used as a building block for synthesizing peptide-PEG conjugates with specific terminal functionalities.

Basic Information

Product Name	Acid-PEG3-NHS Ester
CAS No.	1835759-79-9
Molecular Formula	C15H23NO9
Molecular Weight	361.35 g/mol
Synonyms	NHS-PEG3-COOH; 3-{2-[2-(2,5-Dioxopyrrolidin-1-yl)ethoxy]ethoxy}propanoic Acid; 1-[8-(2-Carboxyethoxy)-3,6-dioxaoctyl]pyrrole-2,5-dione; PEG3-acid-NHS ester; Carboxy-PEG3-NHS Ester; NHS-PEG3-Acid; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(2-hydroxyethoxy)ethoxy]ethoxy]propanoate (related precursor); Acetic acid, 2-[2-[2-[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethoxy]ethoxy]ethoxy]-
EINECS	Contact for details

Quality Control

Our Acid-PEG3-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (H-NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.