Description

Alexa Fluor 647 Maleimide is a high-performance, far-red fluorescent dye derivative designed for site-specific bioconjugation. This compound is essential for researchers and developers requiring stable, bright labeling of thiol-containing biomolecules such as proteins, peptides, and antibodies. It is a critical reagent in advanced life science research, diagnostics, and assay development, enabling sensitive detection and imaging applications.

Application

• Bioconjugation and Protein Labeling: Site-specific covalent attachment to cysteine residues in proteins, antibodies, and peptides for creating fluorescent probes.
• Flow Cytometry: Preparation of bright, photostable antibody-dye conjugates for multi-color cell analysis and immunophenotyping.
• Fluorescence Microscopy & Imaging: Labeling of cellular components for super-resolution microscopy, confocal imaging, and in situ hybridization (FISH) applications.
• Diagnostic Assay Development: Used in the manufacture of fluorescent lateral flow assays, ELISA kits, and other in vitro diagnostic (IVD) components.
• Biomolecular Interaction Studies: Fluorescent tagging for fluorescence polarization (FP), Förster resonance energy transfer (FRET), and other binding assays.
• Nanoparticle and Material Science: Functionalization of nanoparticles, polymers, and surfaces with fluorescent markers for tracking and targeting.

Basic Information

Product Name	Alexa Fluor 647 Maleimide
CAS No.	1825332-75-9
Molecular Formula	C45H51N4O21S63- • 3Na+ (or similar, contact for exact salt form)
Molecular Weight	~1250 g/mol (Contact for details)
Synonyms	AF647 Maleimide; Alexa Fluor® 647 C2 Maleimide; Alexa647 Maleimide; Succinimidyl ester of Alexa Fluor 647 carboxylic acid (precursor); 6-((((2-((3-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)-2,2-dimethyl-1-(3-sulfonatopropyl)-1,3-dihydro-2H-benzo[e]indol-6-yl)sulfonyl)ethyl)amino)carbonyl)amino)-2-((3-((2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)-2,2-dimethyl-1-(3-sulfonatopropyl)-1,3-dihydro-2H-benzo[e]indol-6-yl)sulfonyl)amino)hexanoic acid (sodium salt); Alexa Fluor® 647 maleimide, triethylammonium salt.
EINECS	Contact for details

Quality Control

Our Alexa Fluor 647 Maleimide is manufactured under strict quality systems to ensure batch-to-batch consistency and performance. Each lot is characterized by advanced analytical techniques including HPLC, mass spectrometry, and NMR to confirm identity, purity, and labeling functionality. Certificates of Analysis (COA) detailing spectral properties (Absorption/Emission Maxima, Extinction Coefficient, Degree of Labeling) are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark blue to purple solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 90% (Dye content)
Absorption Maximum (λmax)	650 ± 3 nm
Emission Maximum (λem)	668 ± 3 nm
Extinction Coefficient (ε)	~270,000 cm-1M-1
Solubility	Soluble in DMSO, DMF, water (pH >6)
Maleimide Functionality	Reactive to thiols, confirmed by assay
Moisture Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.