Description

Azido-Peg5-Pfp Ester is a heterobifunctional polyethylene glycol (PEG) linker featuring an azide group at one terminus and a pentafluorophenyl (PFP) ester at the other. This compound is a critical reagent for site-specific bioconjugation and click chemistry applications, enabling the efficient and controlled coupling of biomolecules. It is primarily utilized by researchers and process developers in the pharmaceutical, biotechnology, and diagnostics industries for constructing antibody-drug conjugates (ADCs), modifying peptides and proteins, and developing advanced polymeric materials.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: The PFP ester reacts efficiently with amine groups on antibodies, while the azide allows for subsequent strain-promoted or copper-catalyzed click chemistry with alkyne-bearing cytotoxic payloads.
• Peptide and Protein Modification: Used to install PEG chains (PEGylation) or functional handles onto lysine residues or N-termini of peptides and proteins to enhance solubility, stability, or pharmacokinetics.
• Polymer and Material Science: Serves as a versatile building block for creating functionalized hydrogels, smart polymers, and surface coatings via sequential conjugation reactions.
• Diagnostic Probe Development: Facilitates the labeling of targeting molecules (e.g., antibodies, peptides) with fluorescent dyes or other reporter molecules for imaging and detection assays.
• Solid-Phase Synthesis: Employed as a soluble activating agent for coupling reactions in peptide and oligonucleotide synthesis workflows.
• Crosslinking Agent: Enables the formation of stable amide bonds between amine-containing molecules, useful for creating crosslinked networks or immobilizing biomolecules on surfaces.

Basic Information

Product Name	Azido-Peg5-Pfp Ester
CAS No.	1818294-48-2
Molecular Formula	C24H32F5N3O8
Molecular Weight	609.53 g/mol
Synonyms	Azido-PEG5-PFP ester; 2,3,5,6-Tetrafluoro-4-pentafluorophenyl 2,5,8,11,14-pentaoxa-17-azanonadecan-19-oate; 5-Azido-3,6,9,12,15-pentaoxaheptadecan-1-oic acid 2,3,5,6-tetrafluoro-4-(pentafluorophenyl) ester; PFP-PEG5-Azide; N3-PEG5-PFP ester; Azido-PEG5-PFP Ester; Pentafluorophenyl ester of Azido-PEG5-acid
EINECS	Contact for details

Quality Control

Our Azido-Peg5-Pfp Ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our customers' regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and the PFP ester moiety is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	Colorless to pale yellow oil or solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.