Description

Azido-Peg6-Pfp Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and a pentafluorophenyl (PFP) ester. This compound is a critical reagent for bioorthogonal click chemistry and site-specific bioconjugation, enabling the efficient and stable coupling of biomolecules under mild, aqueous conditions. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostics sectors for applications such as antibody-drug conjugate (ADC) development, peptide modification, and surface functionalization of nanoparticles and biosensors.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via the reactive PFP ester, followed by strain-promoted azide-alkyne cycloaddition (SPAAC) for linker assembly.
• Peptide and Protein Modification: Introduction of azide handles onto peptides or proteins for subsequent click chemistry reactions, enabling labeling, PEGylation, or immobilization.
• Surface Functionalization: Crafting functional surfaces on biosensors, microarrays, or nanoparticles by reacting the PFP ester with surface amines, leaving azide groups for further bioorthogonal coupling.
• Polymer and Material Science: Serving as a versatile building block for synthesizing functionalized hydrogels, smart polymers, and drug delivery systems through sequential click reactions.
• Diagnostic Probe Synthesis: Conjugation of fluorescent dyes, quenchers, or other reporter molecules to targeting ligands (e.g., antibodies, oligonucleotides) for in vitro diagnostics (IVD).
• Chemical Biology Tool Development: Creating activity-based probes or affinity tags for studying protein function, localization, and interactions in living systems.

Basic Information

Product Name	Azido-Peg6-Pfp Ester
CAS No.	1818294-47-1
Molecular Formula	C27H36F5N3O9
Molecular Weight	641.59 g/mol
Synonyms	Azido-PEG6-PFP ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; Pentafluorophenyl 3-[2-[2-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; 1-Azido-3,6,9,12,15,18-hexaoxahenicosan-21-oic acid pentafluorophenyl ester; Azido-PEG6-PFP ester; N3-PEG6-PFP ester; Heterobifunctional PEG crosslinker, Azide-PFP; PFP-PEG6-N3
EINECS	Contact for details

Quality Control

Our Azido-Peg6-Pfp Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR, MS), to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (NMR)	Conforms to structure
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.