Description

Azido-PEG1-NHS Ester is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a click chemistry-ready azide group. This compound is essential for creating stable amide bonds with primary amines, enabling subsequent bioorthogonal conjugation via azide-alkyne cycloaddition. It is a critical tool for researchers and manufacturers in bioconjugation, proteomics, and ADC (Antibody-Drug Conjugate) development, facilitating the precise labeling and modification of biomolecules.

Application

• Bioconjugation and Protein Labeling: For site-specific modification of antibodies, proteins, and peptides by reacting with lysine residues.
• ADC (Antibody-Drug Conjugate) Linker Synthesis: Serves as a key building block for constructing cleavable or non-cleavable linkers between cytotoxic payloads and monoclonal antibodies.
• Surface Functionalization: Used to introduce azide groups onto nanoparticles, biosensors, and microarray surfaces for subsequent click chemistry immobilization of ligands.
• Polymer and Dendrimer Modification: Enables the functionalization of PEG-based polymers and dendritic structures for drug delivery system development.
• Proteomics Research: Aids in the preparation of affinity tags, activity-based probes, and mass spectrometry tags for studying protein function and interaction.
• Diagnostic Reagent Development: Employed in the synthesis of fluorescent, enzymatic, or radioactive probes for in vitro and in vivo imaging.

Basic Information

Product Name	Azido-PEG1-NHS Ester
CAS No.	1807530-06-8
Molecular Formula	C9H12N4O4
Molecular Weight	240.22 g/mol
Synonyms	NHS-PEG1-Azide; 2,5-Dioxopyrrolidin-1-yl 3-azidopropyl carbonate; Azido-PEG1-OSu; 3-Azidopropyl (2,5-dioxopyrrolidin-1-yl) carbonate; PEG1-Azide NHS Ester; N-(3-Azidopropyloxycarbonyloxy)succinimide; Azido-PEG1-NHS Ester; Azido-PEG1-NHS
EINECS	Contact for details

Quality Control

Our Azido-PEG1-NHS Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee traceability and compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.