Description

Cyanine5 Amine CAS NO 1807529-70-9 is a reactive near-infrared fluorescent dye derivative, essential for creating targeted fluorescent probes and conjugates. Its primary value lies in its amine-reactive succinimidyl ester (NHS ester) group, which allows for stable covalent labeling of biomolecules like antibodies, proteins, and peptides. This product is critically needed by researchers and developers in bioconjugation, in vivo imaging, flow cytometry, and diagnostic assay development.

Application

• Bioconjugation and Probe Synthesis: Covalent labeling of proteins, antibodies, oligonucleotides, and other biomolecules for fluorescence-based applications.
• In Vivo and In Vitro Imaging: Used to create targeted imaging agents for near-infrared (NIR) fluorescence microscopy and small animal imaging studies.
• Flow Cytometry & Fluorescence-Activated Cell Sorting (FACS): Labeling cell surface markers or intracellular targets for high-sensitivity detection.
• Diagnostic Assay Development: Key component in developing fluorescent immunoassays (FLISA) and lateral flow tests.
• Drug Delivery & Pharmacokinetics: Tracking the distribution and uptake of therapeutic compounds and nanoparticles.
• Biomaterial & Sensor Functionalization: Incorporating fluorescence into polymers, surfaces, and biosensors for detection purposes.

Basic Information

Product Name	Cyanine5 Amine
CAS No.	1807529-70-9
Molecular Formula	C37H45N3O2+
Molecular Weight	563.78 g/mol (cation)
Synonyms	Cy5 amine; Cy5 NHS ester; Cy5 SE; Sulfo-Cyanine5 NHS ester (note: often associated); 1-(5-Carboxypentyl)-1'-ethyl-3,3,3',3'-tetramethylindocarbocyanine-5,5'-dicarboxylic acid bis(N-succinimidyl ester); Cyanine5 carboxylic acid succinimidyl ester; Cy5 reactive dye; NIR dye NHS ester
EINECS	Contact for details

Quality Control

Our Cyanine5 Amine is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom purity grades and analytical services are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation. Store away from moisture and strong oxidizing agents.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Absorption (λmax)	~649 nm
Emission (λmax)	~670 nm
Extinction Coefficient (ε)	~250,000 L·mol⁻¹·cm⁻¹
Solubility	DMSO, DMF, acetonitrile
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.